Characterization of the lung microbiome and exploration of potential bacterial biomarkers for lung cancer
Characterization of the lung microbiome and exploration of potential bacterial biomarkers for lung cancer
复制标题
肺微生物组的表征和肺癌潜在细菌生物标志物的探索
DOI：
10.21037/tlcr-19-590
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发表时间：
2020-06-01
影响因子：
4
通讯作者：
Gu, Bing
中科院分区：
文献类型：
--
作者：
Cheng, Chen;Wang, Zhifeng;Gu, Bing
背景：新出现的证据表明，肺部微生物群的生态失调可能与肺部疾病的发生有关。然而，肺部微生物群与肺癌之间的相互作用仍不清楚。本研究的目的是评估和比较肺癌和良性肺部疾病之间肺微生物群的分类和衍生功能谱的差异。方法：采集32例肺癌患者和22例肺部良性疾病患者的支气管肺泡灌洗液（BALF）标本，采用16S rRNA扩增子测序进行分析。利用生物信息学方法对获得的序列数据进行深入分析。结果：主坐标分析（principal coordinate analysis, PCoA）显示肺癌组与对照组之间存在显著的分化趋势，肺癌患者肺微生物组的丰富度和均匀度与肺部良性疾病患者基本相似。肺癌组与对照组相比，TM7门和6个属（c:TM7-3、Capnocytophaga、Sediminibacterium、Gemmiger、Blautia和Oscillospira）丰富(调整P
Background: Emerging evidence has suggested that dysbiosis of the lung microbiota may be associated with the development of lung diseases. However, the interplay between the lung microbiome and lung cancer remains unclear. The aim of the present study was to evaluate and compare differences in taxonomic and derived functional profiles in the lung microbiota between lung cancer and benign pulmonary diseases.Methods: Bronchoalveolar lavage fluid (BALF) samples were collected from 32 patients with lung cancer and 22 patients with benign pulmonary diseases, and further analyzed by 16S rRNA amplicon sequencing. The obtained sequence data were deeply analyzed by bioinformatics methods.Results: A significant differentiation trend was observed between the lung cancer and control groups based on principal coordinate analysis (PCoA), while richness and evenness in the lung microbiome of lung cancer patients generally resembled those of patients with benign pulmonary diseases. Phylum TM7 and six genera (c:TM7-3, Capnocytophaga, Sediminibacterium, Gemmiger, Blautia and Oscillospira) were enriched in the lung cancer group compared with the control group (adjust P