Investigation of cytotoxicity of negative control peptides versus bioactive peptides on skin cancer and normal cells: a comparative study
Investigation of cytotoxicity of negative control peptides versus bioactive peptides on skin cancer and normal cells: a comparative study
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DOI：
10.4155/fmc.12.98
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发表时间：
2012-08-01
影响因子：
4.2
通讯作者：
Istivan, Taghrid S.
中科院分区：
文献类型：
--
作者：
Almansour, Nahlah M.;Pirogova, Elena;Istivan, Taghrid S.
背景：共振识别模型-粘液瘤病毒(RRM-MV)是粘液瘤病毒MV-T5蛋白的生物活性多肽类似物，由RRM计算设计。本研究以四种阴性对照多肽为对照，研究了RRM-MV对人皮肤癌和正常细胞的体外抗癌作用。结果与讨论：定量和定性评价RRM-MV与阴性对照多肽对细胞的影响。RRM-MV治疗能够诱导癌细胞死亡，而不会对正常细胞产生类似的影响。然而，阴性对照多肽对皮肤癌和正常细胞没有毒性作用。所有多肽对人红细胞均无影响。结论：RRM可用于设计治疗性抗癌肽。
Background: Resonant recognition model-myxoma virus (RRM-MV), a bioactive peptide analogue for myxoma virus MV-T5 protein, was computationally designed by the RRM. In this study, the anticancer effects of RRM-MV were assessed in vitro against four negative control peptides on human skin cancer and normal cells. Results & discussion: The effects of RRM-MV versus negative control peptides on cells were evaluated by quantitative and qualitative assays. The RRM-MV treatment was able to induce cell death in cancer cells without triggering similar effects on normal cells. However, the negative control peptides produced no toxic effects on skin cancer and normal cells. No effects on human erythrocytes were detected when treated with all peptides. Conclusion: It is suggested that the RRM can be applied to design therapeutic anticancer peptides.