Localization of polysaccharides in isolated and intact cuticles of eucalypt, poplar and pear leaves by enzyme-gold labelling
Localization of polysaccharides in isolated and intact cuticles of eucalypt, poplar and pear leaves by enzyme-gold labelling
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DOI：
10.1016/j.plaphy.2013.12.023
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发表时间：
2014-03-01
影响因子：
6.5
通讯作者：
Gil, Luis
中科院分区：
文献类型：
--
作者：
Guzman, Paula;Fernandez, Victoria;Gil, Luis
角质层多糖的存在和特性已被证明是通过染色和光谱方法，但它们在角质层中的位置仍然不清楚。此外，根据流行的模型，多糖被认为局限于角质层，而不存在于角质层中。为了深入了解角质层的超微结构，集中在多糖，纤维素和果胶已被确定，并通过酶金标记（Au-纤维素酶，EC 3.2.1.4，和-果胶酶，EC 3.2.1.15）和透射电子显微镜（TEM）的手段定位在分离和完整的近轴叶角质层的横切面蓝桉，白杨x canescens和梨。观察了角质层与其下细胞壁之间的界面结构对酶法分离叶角质层过程的影响。纤维素和果胶首次在酶促分离的角质层中被检测到，有时出现在表皮蜡层下面。多糖在完整和孤立的叶表皮中的位置和存在可能具有多重意义，例如当估计植物器官和周围环境之间物质的双向运输时，或者当解释器官个体发育时。在植物本体论和生理生态学的背景下讨论的结果。(C)2014年Elsevier Masson SAS。All rights reserved.
The presence and characteristics of cuticle polysaccharides have been demonstrated by staining and spectroscopic methods, but their location in the cuticle remains unclear. Furthermore, according to the prevailing model, polysaccharides are believed to be restricted to the cuticular layer and absent in the cuticle proper. With the aim of gaining insight into cuticular ultra-structure focussing on polysaccharides, cellulose and pectins have been identified and located in the transversal sections of isolated and intact adaxial leaf cuticles of Eucalyptus globulus, Populus x canescens and Pyrus communis by means of enzyme gold-labelling (Au-cellulase, EC 3.2.1.4, and -pectinase, EC 3.2.1.15) and transmission electron microscopy (TEM). The structure of the interface between the cuticle and the cell wall underneath was observed to influence the process of enzymatic isolation of leaf cuticles. Cellulose and pectins were detected for the first time in enzymatically isolated cuticles, sometimes appearing closely underneath the epicuticular wax layer. The location and presence of polysaccharides in intact and isolated leaf cuticles may have multiple implications, such as when estimating the bi-directional transport of substances between plant organs and the surrounding environment, or when interpreting organ ontogeny. The results are discussed within a plant ontological and ecophysiological context. (C) 2014 Elsevier Masson SAS. All rights reserved.