Bromodomain-containing Proteins BRD1, BRD2, and BRD13 are Core Subunits of SWI/SNF Complexes and are Vital for their Genomic Targeting in Arabidopsis.
Bromodomain-containing Proteins BRD1, BRD2, and BRD13 are Core Subunits of SWI/SNF Complexes and are Vital for their Genomic Targeting in Arabidopsis.
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DOI：
10.1016/j.molp.2021.03.018
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发表时间：
2021-03
期刊：
影响因子：
27.5
通讯作者：
Yaoguang Yu;Wei Fu;Jianqu Xu;Y. Lei;Xin Song;Zhenwei Liang;Tao Zhu;Yuhui Liang;Yuanhao Hao;Liangbing Yuan;Chenlong Li
中科院分区：
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作者：
Yaoguang Yu;Wei Fu;Jianqu Xu;Y. Lei;Xin Song;Zhenwei Liang;Tao Zhu;Yuhui Liang;Yuanhao Hao;Liangbing Yuan;Chenlong Li
开关缺陷型/蔗糖非发酵型（SWI/SNF）染色质重塑复合物是一种多亚基机器，在染色质结构和基因表达调控中发挥重要作用。然而，SWI/SNF复合物识别其在植物中的靶基因座的机制还不完全清楚。在这里，我们表明，拟南芥abromodomain含有蛋白质BRD 1，BRD 2，和BRD 13的SWI/SNF复合物的核心亚基和SWI/SNF基因组靶向的关键。这三个BRD直接与多个SWI/SNF亚基相互作用，包括BRAHMA（BRM）催化亚基。对brd 1 brd 2 brd 13三重突变体的表型和转录组学分析显示，这些BRD在很大程度上冗余地控制基因表达和发育过程，这些过程也受BRM的调控。全基因组占有率分析表明，这三种BRD与BRM广泛共定位在染色质上。三个BRD基因的功能同时丧失导致BRM蛋白水平降低和BRM在整个基因组染色质上的占据率降低。此外，我们证明了BRD的溴结构域对于SWI/SNF复合物的BRD亚基到其靶位点的基因组靶向是必需的。总的来说，这些结果表明，BRD 1，BRD 2和BRD 13是SWI/SNF复合物的核心亚基，并揭示了它们在促进植物中含有BRM的SWI/SNF复合物的基因组靶向中的生物学作用。
Switch defective/sucrose non-fermentable (SWI/SNF) chromatin remodeling complexes are multi-subunit machines that play vital roles in the regulation of chromatin structure and gene expression. However, the mechanisms by which SWI/SNF complexes recognize their target loci in plants are not fully understood. Here, we show that theArabidopsis thalianabromodomain-containing proteins BRD1, BRD2, and BRD13 are core subunits of SWI/SNF complexes and critical for SWI/SNF genomic targeting. These three BRDs interact directly with multiple SWI/SNF subunits, including the BRAHMA (BRM) catalytic subunit. Phenotypic and transcriptomic analyses of thebrd1 brd2 brd13triple mutant revealed that these BRDs act largely redundantly to control gene expression and developmental processes that are also regulated by BRM. Genome-wide occupancy profiling demonstrated that these three BRDs extensively colocalize with BRM on chromatin. Simultaneous loss of function of threeBRDgenes results in reduced BRM protein levels and decreased occupancy of BRM on chromatin across the genome. Furthermore, we demonstrated that the bromodomains of BRDs are essential for genomic targeting of the BRD subunits of SWI/SNF complexes to their target sites. Collectively, these results demonstrate that BRD1, BRD2, and BRD13 are core subunits of SWI/SNF complexes and reveal their biological roles in facilitating genomic targeting of BRM-containing SWI/SNF complexes in plants.