Tooth loss causes spatial cognitive impairment in rats through decreased cerebral blood flow and increased glutamate
Tooth loss causes spatial cognitive impairment in rats through decreased cerebral blood flow and increased glutamate
复制标题
牙齿缺失通过减少脑血流量和增加谷氨酸导致大鼠空间认知障碍。
DOI：
10.1016/j.archoralbio.2019.05.004
复制
发表时间：
2019-06-01
影响因子：
3
通讯作者：
Jiang, Qingsong
中科院分区：
文献类型：
--
作者：
Luo, Bin;Pang, Qian;Jiang, Qingsong
目的：牙齿脱落不仅会对口腔功能造成损害，还会导致认知障碍。以前的研究已经报道咀嚼可以增加CBF，而CBF在认知功能中起着重要作用。目前尚不清楚牙齿脱落是否会通过减少脑脑容量而导致认知障碍。本研究旨在探讨大鼠脱牙后脑血流、谷氨酸浓度、神经元凋亡相关bax /Bcl-2和Caspase-3 mRNA及海马锥体细胞表达的变化及行为变化。设计：拔牙12周后，采用Morris水迷宫测试大鼠上颌磨牙的空间学习记忆能力，采用ASL-MRI检测脑血流，HPLC检测谷氨酸浓度；测定神经元凋亡相关的Bax/Bcl-2和Caspase-3 mRNA的表达以及CA1区锥体细胞的数量。结果：脱牙大鼠在Morris水迷宫中表现出空间认知障碍，CBF下降，海马谷氨酸水平升高，bax /Bcl-2和Caspase-3 mRNA表达升高；CA1区锥体细胞数量减少。结论：这些研究结果表明，牙齿缺失导致大鼠空间认知障碍，其潜在机制可能与脑血流减少和海马谷氨酸水平升高有关。
Objective: The loss of teeth not only causes damage to oral function but also is associated with cognitive impairment. Previous studies have reported that chewing can increase CBF, and CBF plays an important role in cognitive function. Whether the loss of teeth can lead to cognitive impairment by reducing CBF is unclear. This study aimed to investigate the changes in CBF, glutamate concentration, the expression of neuronal apoptosis-relatedBax/Bcl-2 and Caspase-3 mRNA and pyramidal cells in the hippocampus, as well as behavioral changes after tooth loss in rats.Design: Twelve weeks after the extraction of all maxillary molars in rats, their spatial learning and memory were tested by the Morris water maze, the CBF was detected by ASL-MRI and glutamate concentration was detected by HPLC; the expression of neuronal apoptosis-related Bax/Bcl-2 and Caspase-3 mRNA and the number of pyramidal cells in the CA1 region were also measured.Results: Rats with tooth loss exhibited spatial cognitive impairment in the Morris water maze, decreased CBF, increased glutamate levels andBax/Bcl-2 and Caspase-3 mRNA expression in the hippocampus; the number of pyramidal cells in the CA1 region were also reduced.Conclusions: These findings suggest that the loss of teeth causes spatial cognitive impairment in rats and that the underlying mechanism might be associated with a decrease in CBF and an increase in the glutamate level in the hippocampus.