Functional and molecular expression of PACAP/VIP receptors in the rat retina
Functional and molecular expression of PACAP/VIP receptors in the rat retina
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DOI：
10.1016/s0169-328x(97)00335-5
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发表时间：
1998-02-01
期刊：
影响因子：
--
通讯作者：
Cavallaro, S
中科院分区：
文献类型：
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作者：
D'Agata, V;Cavallaro, S
垂体腺苷酸环化酶激活多肽（PACAP）和血管活性肠多肽（VIP）的受体结合位点，正偶联腺苷酸环化酶，先前已被描述在不同的哺乳动物物种的视网膜。在本研究中，我们测定了大鼠视网膜中PACAP/VIP受体变体的mRNA表达，并研究了它们与磷脂酶C以及腺苷酸环化酶的偶联。两种形式的PACAP，PACAP 27和PACAP 38，诱导cAMP和[H-3]肌醇单磷酸水平的剂量依赖性（1-100 nM）增加，而VIP刺激，具有较低的效力和功效，cAMP的形成。大鼠视网膜中的逆转录-PCR分析检测到I型（PACAP-R和PACAP-HOP剪接变体）和II型（VIP-1和VIP-2）受体-mRNA。这些数据表明，PACAP和VIP可能与多种受体亚型相互作用，并激活一个（VIP）或两个（PACAP）的信号转导机制在大鼠视网膜。(C)1998年Elsevier Science B.V.
Receptor binding sites for pituitary adenylate cyclase activating polypeptide (PACAP) and vasoactive intestinal polypeptide (VIP), positively coupled to adenylate cyclase, have been previously described in the retina of different mammalian species. In the present study, we determined the mRNA expression of PACAP/VIP receptor variants in the rat retina and investigated their coupling to phospholipase C in addition to adenylate cyclase. The two forms of PACAP, PACAP27 and PACAP38, induced a dose-dependent (1-100 nM) increase of cAMP and [H-3]inositol monophosphate levels, whereas VIP stimulated, with lower potency and efficacy, cAMP formation only. Reverse transcription-PCR analysis in the rat retina detected both type-I (PACAP-R and PACAP-HOP splice variants) and type-II(VIP-l and -2) receptor-mRNAs. These data indicate that PACAP and VIP may interact with multiple receptor subtypes and activate one (VIP) or two (PACAP) signal transduction mechanisms in the rat retina. (C) 1998 Elsevier Science B.V.