Expression of chromatin remodeling factors during neural differentiation
Expression of chromatin remodeling factors during neural differentiation
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DOI：
10.1093/oxfordjournals.jbchem.a002834
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发表时间：
2001-01-01
影响因子：
2.7
通讯作者：
Iijima, S
中科院分区：
文献类型：
--
作者：
Machida, Y;Murai, K;Iijima, S
酵母 SWI/SNF 复合物参与转录调节过程中染色质结构的重塑。该复合物的关键亚基之一称为 SWI2/SNF2，具有 DNA 依赖性 ATP 酶活性。最近鉴定出两种不同类型的酵母 SWI2/SNF2 哺乳动物同源物，称为 BRM 和 BRG1。它们在结构上非常相似，但功能却截然不同。我们研究了体外培养的神经前体细胞（NPC）分化过程中 BRM 和 BRG1 的表达。 BRM在NPC中的表达非常低，并且在分化为神经元和星形胶质细胞期间被诱导至高水平，相反，BRG1在整个分化过程中持续表达。这些现象在P19胚胎癌细胞向神经细胞的分化中也观察到。免疫细胞化学分析显示，即使在未分化的巢蛋白阳性细胞中，BRM 也开始表达。这些结果表明BRM可能在神经细胞分化中发挥重要作用。
The yeast SWI/SNF complex is involved in remodeling of chromatin structure during transcriptional modulation. One of the key subunits of this complex, called SWI2/SNF2, has a DNA-dependent ATPase activity. Two different types of mammalian homolog of yeast SWI2/SNF2, called BRM and BRG1, were recently identified. They are closely similar in structure but have distinct functions. We investigated the expression of BRM and BRG1 during differentiation of neural precursor cells (NPCs) cultured in vitro. The expression of BRM was very low in NPCs and was induced to a high level during differentiation to neurons and astrocytes, In contrast, BRG1 was constantly expressed throughout differentiation. These phenomena were also observed in differentiation of P19 embryonal carcinoma cells to neural cells. Immunocytochemical analyses revealed that the expression of BRM started even in the undifferentiated nestin-positive cells. These results indicate that BRM may have an important role in neural cell differentiation.