Investigating the presence of neutrophil extracellular traps in cutaneous lesions of different subtypes of lupus erythematosus
Investigating the presence of neutrophil extracellular traps in cutaneous lesions of different subtypes of lupus erythematosus
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DOI：
10.1111/exd.14040
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发表时间：
2019-09-29
影响因子：
3.6
通讯作者：
Nassar, Dany
中科院分区：
文献类型：
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作者：
Safi, Remi;Al-Hage, Jana;Nassar, Dany
中性粒细胞胞外陷阱（NET）与系统性红斑狼疮（SLE）的发病机制有关。然而，人们对 NET 对皮肤狼疮的影响知之甚少。在这个由 30 名患者组成的病例系列中，我们比较了不同狼疮亚型（5 例盘状狼疮或 DLE，5 例亚急性皮肤狼疮或 SCLE，11 例急性皮肤 SLE，7 例狼疮性脂膜炎，2 例冻疮）皮损中产生 NET 的中性粒细胞数量。使用针对中性粒细胞颗粒的抗体（弹性蛋白酶、髓过氧化物酶、PR-3 蛋白和瓜氨酸组蛋白 3）对福尔马林固定石蜡包埋的皮肤活检进行免疫荧光检查。进行二氢乙锭染色以检测活性氧 (ROS)，即已知的 NET 诱导剂。在不同亚型的皮肤狼疮以及 SLE 的皮肤病变中均检测到了 NET。与 SCLE (5%) 和冻疮 (0%) 相比，狼疮性脂膜炎 (49%)、急性皮肤 SLE (41%) 和 DLE (32%) 中产生 NET 的中性粒细胞数量显着更高。这表明 NET 可能与更多的组织损伤和疤痕相关。在狼疮的不同皮肤亚型中观察到 ROS，与 NET 无关。
Neutrophil extracellular traps (NETs) are implicated in the pathogenesis of systemic lupus erythematosus (SLE). However, little is known about the implication of NETs in cutaneous lupus. In this case series of 30 patients, we compared the amounts of neutrophils producing NETs in cutaneous lesions of different subtypes of lupus (5 with discoid lupus or DLE, 5 with subacute cutaneous lupus or SCLE, 11 with acute cutaneous SLE, 7 with lupus panniculitis and 2 with chilblains). Immunofluorescence was performed on formalin-fixed paraffin-embedded skin biopsies using antibodies against neutrophilic granules (elastase, myeloperoxidase, PR-3 proteins and citrullinated histone 3). Dihydroethidium staining was performed to detect reactive oxygen species (ROS), known inducers of NETs. NETs were detected in the different subtypes of cutaneous lupus as well as in cutaneous lesions of SLE. The amounts of neutrophils producing NETs were significantly higher in lupus panniculitis (49%), acute cutaneous SLE (41%) and DLE (32%), in comparison with SCLE (5%) and chilblains (0%). This suggests that NETs might be associated with more tissue damage and scarring. ROS were observed in the different cutaneous subtypes of lupus independent of NETs.