Gene silencing by RNA interference in the house dust mite, Dermatophagoides pteronyssinus
Gene silencing by RNA interference in the house dust mite, Dermatophagoides pteronyssinus
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DOI：
10.1016/j.mcp.2015.07.008
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发表时间：
2015-12-01
影响因子：
3.3
通讯作者：
Burgess, Stewart T. G.
中科院分区：
文献类型：
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作者：
Marr, Edward J.;Sargison, Neil D.;Burgess, Stewart T. G.
这是欧洲屋尘螨（Dermatophagoides pteronyssinus，Trouessart，1897）通过RNA干扰（RNAi）进行基因沉默的首次报道。使用一种非侵入性的浸泡方法，首先开发的蜜蜂螨，狄斯瓦螨，一个显着减少的表达D。在编码DpGST-mul的双链RNA中浸泡过夜后，获得了屋尘螨谷胱甘肽-S-转移酶μ-1类酶（DpGST-mul）。虽然在沉默后没有观察到有害的表型变化，但这种技术现在可以用于解决基本的生理问题并评估沉默D的潜在治疗益处。屋尘螨靶基因在选定的高人螨界面的国内情况下。(C)2015爱思唯尔有限公司版权所有。
This is the first report of gene silencing by RNA interference (RNAi) in the European house dust mite, Dermatophagoides pteronyssinus, Trouessart, 1897. Using a non-invasive immersion method first developed for the honey bee mite, Varroa destructor, a significant reduction in the expression of D. pteronyssinus glutathione-S-transferase mu-class 1 enzyme (DpGST-mul) was achieved following overnight immersion in double stranded RNA encoding DpGST-mul. Although no detrimental phenotypic changes were observed following silencing, this technique can now be used to address fundamental physiological questions and assess the potential therapeutic benefit in silencing D. pteronyssinus target genes in selected domestic situations of high human-mite interface. (C) 2015 Elsevier Ltd. All rights reserved.