Nestin expression in high-grade osteosarcomas and its clinical significance.
Nestin expression in high-grade osteosarcomas and its clinical significance.
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DOI：
10.3892/or.2012.1687
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发表时间：
2012-05
期刊：
影响因子：
4.2
通讯作者：
I. Zambo;M. Hermanová;D. Adámková Krákorová;P. Múdry;K. Zitterbart;M. Kyr;K. Veselý;J. Štěrba;R. Veselska
中科院分区：
文献类型：
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作者：
I. Zambo;M. Hermanová;D. Adámková Krákorová;P. Múdry;K. Zitterbart;M. Kyr;K. Veselý;J. Štěrba;R. Veselska
巢蛋白已在多种恶性肿瘤中检测到，并且其表达与某些肿瘤的晚期分级相关。本研究的目的是检测巢蛋白在高级别骨肉瘤中的表达，并确定其预后价值。采用免疫组化和免疫荧光法，我们评估了巢蛋白表达在45例高级别骨肉瘤患者的肿瘤组织样本。在两种方法中，nestin阳性肿瘤细胞的频率分为三类（免疫组化为1+、2+和3+;免疫荧光为1F+、2F+和3F+），并对临床病理相关性进行统计学评价和分析。巢蛋白阳性的肿瘤细胞检测在所有的检查骨肉瘤使用免疫组化和免疫荧光，虽然无疑是阳性肿瘤细胞的比例在个别样品从几个巢蛋白阳性的肿瘤细胞扩散巢蛋白阳性。免疫荧光检测到高水平的巢蛋白表达（2F+和3F+）与较差的临床结局相关（OS，p=0.031; EFS，p<0.001）。然而，高水平的巢蛋白表达的免疫组化测定的患者生存率较短的趋势，但没有达到统计学意义。尽管通过免疫荧光法评估的高水平巢蛋白表达患者的生存率明显较短，但巢蛋白似乎并不代表一种强有力的预后标志物，其上级于常规方法。
Nestin has been detected in various malignancies and its expression correlates with advanced grade in some neoplasms. The aim of this study was to examine nestin expression in high-grade osteosarcomas and to determine its prognostic value. Using immunohistochemistry and immunofluorescence, we evaluated nestin expression in tumor tissue samples from 45 patients with high-grade osteosarcomas. In both methods, the frequency of nestin-positive tumor cells was classified into three categories (1+, 2+ and 3+ for immunohistochemistry; 1F+, 2F+ and 3F+ for immunofluorescence) and clinicopathological correlations were statistically evaluated and analyzed. Nestin-positive tumor cells were detected in all of the examined osteosarcomas using both immunohistochemistry and immunofluorescence, although the proportion of undoubtedly positive neoplastic cells varied in individual samples from a few nestin-positive tumor cells to diffuse nestin positivity. High levels of nestin expression detected by immunofluorescence (2F+ and 3F+) were associated with worse clinical outcomes (OS, p=0.031; EFS, p<0.001). However, high levels of nestin expression as measured by immunohistochemistry trended towards shorter patient survival rates but did not reach statistical significance. Despite significantly shorter survival rates observed in patients with high levels of nestin expression assessed by immunofluorescence, nestin does not seem to represent a powerful prognostic marker that would be superior to conventional methods.