Dynamics of bacterial microbiota during lignocellulosic waste composting: Studies upon its structure, functionality and biodiversity
Dynamics of bacterial microbiota during lignocellulosic waste composting: Studies upon its structure, functionality and biodiversity
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DOI：
10.1016/j.biortech.2014.10.123
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发表时间：
2015-01-01
影响因子：
11.4
通讯作者：
Moreno, J.
中科院分区：
文献类型：
--
作者：
Lopez-Gonzalez, J. A.;Suarez-Estrella, F.;Moreno, J.
在三个复制的堆肥堆中进行的强化隔离计划可以识别堆肥微生物组的常驻和瞬时成分。通过对其 16S rRNA 基因进行部分测序，分离、酶促表征和鉴定了 4000 多种细菌菌株。虽然在整个过程中在中温条件下分离的微生物很突出，但高温阶段的总计数最高，并且孢子形成细菌在堆肥的生物氧化阶段占主导地位。大多数分离株都表现出与聚合物材料降解相关的酶促能力，并且由于这些活性，可以为整个堆肥微生物群提供更多可溶性化合物。大部分分离株表现出耐热性，因为它们在中温和高温阶段被检测到。分离菌株属于 187 个细菌种类。堆肥中心阶段的生物多样性更高，嗜温、嗜热和冷却阶段的物种占 50%。 (C) 2014 Elsevier Ltd. 保留所有权利。
An intensive isolation program carried out in three replicated composting piles allowed the identification of the resident and transient components of the composting microbiome. More than 4000 bacterial strains were isolated, enzymatically characterized and identified by partial sequencing of their 16S rRNA gene. While microorganisms isolated under mesophilic conditions were prominent throughout the process, thermophilic stages gathered the highest total counts and spore-forming bacteria prevailed at the bio-oxidative phase of composting. Enzymatic capabilities related to the degradation of polymeric materials were exhibited by most of the isolates and as a result of these activities, more soluble compounds could be made available to the entire composting microbiota. A high proportion of isolates showed to be thermotolerant as they were detected at mesophilic and thermophilic phases. Isolated strains belonged to 187 bacterial species. Biodiversity was greater at the central stages of composting and mesophilic, thermophilic and cooling phases shared 50% of species. (C) 2014 Elsevier Ltd. All rights reserved.