Collagen distribution in the human vitreoretinal interface
Collagen distribution in the human vitreoretinal interface
复制标题
DOI：
10.1167/iovs.07-1456
复制
发表时间：
2008-09-01
影响因子：
4.4
通讯作者：
Los, Leonoor I.
中科院分区：
文献类型：
--
作者：
Ponsioen, Theodorus L.;van Luyn, Marja J. A.;Los, Leonoor I.
目的.为了评估在人供体眼中后极、赤道和赤道前区I至VII、IX、XI和XVIII型胶原的存在，因为胶原是有助于玻璃体视网膜界面处的玻璃体视网膜粘附的重要大分子。采用新鲜分离的赤道人视网膜切除术样本进行逆转录-聚合酶链反应检测上述胶原蛋白的mRNA。此外，将人供体眼睛和赤道视网膜切除术样本包埋在石蜡中，用抗胶原抗体染色，并通过光学显微镜（LM）进行评价。视网膜切除术样品表达所有测试的胶原类型的mRNA。通过LM，玻璃体皮质对II、V、IX和XI型胶原蛋白呈阳性。在供体眼和视网膜切除术样本的所有三个区域中，内界膜（ILM）显示IV、VI和XVIII型;大多数标本中视网膜血管系统对I至VI和XVIII型呈阳性;视网膜层显示VII型浓缩斑点。此外，VII型在视网膜层的密度和分布向后极增加。I至V、IX、XI和XVIII型胶原蛋白的染色模式证实了先前的观察结果。重要的新发现包括在ILM中存在VI型和在视网膜的几个层中存在VII型。两种胶原都可以锚基质成分，VI型胶原可能参与玻璃体视网膜附着。此外，人视网膜切除术样本中胶原mRNA的存在可能是视网膜细胞产生出生后胶原的指示。
PURPOSE. To evaluate the presence of collagen types I to VII, IX, XI, and XVIII at the posterior pole, the equator and the preequatorial area in human donor eyes, since collagens are important macromolecules that contribute to vitreoretinal adhesion at the vitreoretinal interface.METHODS. Freshly isolated human retinectomy samples from the equator were used for reverse transcription-polymerase chain reaction to detect mRNA of the above-mentioned collagens. In addition, human donor eyes and equatorial retinectomy samples were embedded in paraffin, stained with antibodies against the collagens and evaluated by light microscopy (LM).RESULTS. Retinectomy samples expressed mRNA of all tested collagen types. By LM, vitreous cortex was positive for collagen types II, V, IX, and XI. In all three regions within the donor eyes and in the retinectomy samples, the internal limiting membrane (ILM) showed types IV, VI, and XVIII; the retinal vasculature was positive for types I to VI and XVIII in most specimens; and the retinal layers showed condensed spots of type VII. In addition, type VII increased in density and in distribution over the retinal layers toward the posterior pole.CONCLUSIONS. Staining patterns of collagen types I to V, IX, XI, and XVIII confirmed previous observations. Important new findings include the presence of type VI in the ILM and type VII in several layers of the retina. Both collagens can anchor matrix components, and type VI could be involved in vitreoretinal attachment. Furthermore, the presence of collagen mRNA in human retinectomy samples may be an indication of postnatal collagen production by retinal cells.