The regulated expression of cell cycle-related proteins as B-lymphocytes enter and progress through the G1 cell cycle stage following delivery of complete versus partial activation stimuli.
The regulated expression of cell cycle-related proteins as B-lymphocytes enter and progress through the G1 cell cycle stage following delivery of complete versus partial activation stimuli.
复制标题
当 B 淋巴细胞在给予完全激活刺激或部分激活刺激后进入并进入 G1 细胞周期阶段时，细胞周期相关蛋白的表达受到调节。
DOI：
10.1016/s0161-5890(96)00065-x
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发表时间：
1996
影响因子：
3.6
通讯作者：
Snow,EC
中科院分区：
文献类型：
--
作者：
Reid,S;Snow,EC
静止的b细胞缺乏周期蛋白D和E，同时组成性地表达低水平的cdk4和cdk2。接受完全生长刺激的b细胞在刺激后10小时表达周期蛋白D2, 10 - 24小时表达周期蛋白E，同时增加cdk4和cdk2的蛋白水平。接受部分生长刺激的b细胞在不通过g1限制点的情况下进入g1，并在不积累cyclin D2蛋白的情况下短暂地增加cyclin D2 mRNA水平。在缺乏cyclin D2积累的情况下，cdk4不被激活，cyclin E不表达。这些结果表明，负责移动b细胞通过g1限制点的信号影响细胞周期蛋白D2蛋白积累水平。讨论了这些结果可能产生的影响。
Resting B-cells lack both cyclins D and E while constitutively expressing low levels of cdk4 and cdk2. B-cells receiving a complete growth stimulus express cyclin D2 by 10 hr and cyclin E by 10–24 hr poststimulation while increasing their protein levels of cdk4 and cdk2. B-cells receiving partial growth stimuli move into G1without passing the G1restriction point and transiently increase cyclin D2 mRNA levels without accumulating cyclin D2 protein. In the absence of cyclin D2 accumulation, cdk4 is not activated, and cyclin E is not expressed. These results suggest that signals responsible for moving B-cells through the G1restriction point impact at the level of cyclin D2 protein accumulation. The possible implications of these results are discussed.