INTERACTION OF THE P53-REGULATED PROTEIN GADD45 WITH PROLIFERATING CELL NUCLEAR ANTIGEN
INTERACTION OF THE P53-REGULATED PROTEIN GADD45 WITH PROLIFERATING CELL NUCLEAR ANTIGEN
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DOI：
10.1126/science.7973727
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发表时间：
1994-11-25
期刊：
影响因子：
56.9
通讯作者：
FORNACE, AJ
中科院分区：
文献类型：
--
作者：
SMITH, ML;CHEN, IT;FORNACE, AJ
GADD 45是一种广泛表达的哺乳动物基因，由DNA损伤和某些其他应激诱导。与另一个p53调节基因p21（WAF 1/CIP 1）一样，GADD 45与生长抑制相关，其产物与细胞周期蛋白依赖性激酶（Cdk）和增殖细胞核抗原（PCNA）结合。Gadd 45被发现与PCNA结合，PCNA是Cdk复合物的正常组分，也是参与DNA复制和修复的蛋白质。Gadd 45在体外刺激DNA切除修复，抑制细胞进入S期。这些结果确立了GADD 45作为p53依赖性细胞周期检查点和DNA修复之间的联系。
GADD45 is a ubiquitously expressed mammalian gene that is induced by DNA damage and certain other stresses. Like another p53-regulated gene, p21(WAF1/CIP1), whose product binds to cyclin-dependent kinases (Cdk's) and proliferating cell nuclear antigen (PCNA), GADD45 has been associated with growth suppression. Gadd45 was found to bind to PCNA, a normal component of Cdk complexes and a protein involved in DNA replication and repair. Gadd45 stimulated DNA excision repair in vitro and inhibited entry of cells into S phase. These results establish GADD45 as a link between the p53-dependent cell cycle checkpoint and DNA repair.