Effect of TiO2 nanoparticles and multiwall carbon nanotubes on the freshwater diatom Nitzschia frustulum: evaluation of growth, cellular components and morphology
Effect of TiO2 nanoparticles and multiwall carbon nanotubes on the freshwater diatom Nitzschia frustulum: evaluation of growth, cellular components and morphology
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TiO2 纳米颗粒和多壁碳纳米管对淡水硅藻截头菱形藻的影响：生长、细胞成分和形态的评估
DOI：
10.1080/02757540.2018.1528240
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发表时间：
2019
影响因子：
2.5
通讯作者：
Fan Yawen
中科院分区：
文献类型：
--
作者：
Jia Kun;Sun Chenglong;Wang Yulin;Li Xin;Mu Weijie;Fan Yawen
摘要各种纳米颗粒对水生生物可能产生的影响应引起高度重视。淡水硅藻是浮游植物的重要组成部分，在环境中纳米颗粒暴露的生物评估中发挥着关键作用。在这项研究中，细胞生长被TiO 2-NPs和MWCNTs抑制，TiO 2-NPs和MWCNTs对截头菱形藻的24 h EC 50值分别为20.75和24.64 mg L−1。 TiO 2-NPs处理后，叶绿素a含量显著降低，N.在较低浓度下，MWCNTs处理明显增加了细胞的截头体。在N.在TiO 2-NP和MWCNT暴露之后的平截头体。MDA含量显着诱导的TiO 2-NPs在较低浓度的24和48小时的曝光;同时，它增加了在所有测试的浓度在24小时多壁碳纳米管曝光。 TiO 2-NPs处理72和96 h可诱导SOD酶活性，而多壁碳纳米管处理96 h可增加SOD酶活性。 平截头体扫描电子显微镜结果表明，N。经TiO_2-NPs处理后，硅藻细胞发生了明显的变形。结果表明，硅藻对NPs暴露后的生理生化反应具有种特异性，并与暴露浓度和时间有关。
ABSTRACT Great attention should be paid to the possible impacts of various nanoparticles on aquatic organism. Freshwater diatoms are essential components of phytoplankton and play a critical role in bioassessment of nanoparticle exposure in the environment. In this study, cell growth was inhibited by TiO2-NPs and MWCNTs, the 24 h EC50 values of TiO2-NPs and MWCNTs to Nitzschia frustulum were 20.75 and 24.64 mg L−1, respectively. Significant decreases of Chl a content after TiO2-NPs exposures were detected and the Chl a content of N. frustulum was obviously increased by MWCNTs treatment at lower concentration. The ROS was detected in N. frustulum after TiO2-NPs and MWCNTs exposures. The MDA content was significantly induced by TiO2-NPs at lower concentrations of 24 and 48 h exposure; meanwhile, it increased at all tested concentrations at 24 h MWCNTs exposure. The SOD enzyme was induced by 72 and 96 h TiO2-NPs exposure, and increased by MWCNTs treatment at 96 h in N. frustulum. Scanning electron microscopy results revealed that N. frustulum had obvious cell deformation after TiO2-NPs treatment. The result showed that the physiological and biochemical response mechanisms after NPs exposure of diatom were species-specific, and in relation to the exposure concentration and time.