Upregulation of microRNA-203 is associated with advanced tumor progression and poor prognosis in epithelial ovarian cancer
Upregulation of microRNA-203 is associated with advanced tumor progression and poor prognosis in epithelial ovarian cancer
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DOI：
10.1007/s12032-013-0681-x
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发表时间：
2013-09-01
期刊：
影响因子：
3.4
通讯作者：
Xiang, Wei
中科院分区：
文献类型：
--
作者：
Wang, Shaosheng;Zhao, Xiaohong;Xiang, Wei
具有肿瘤抑制或促进活性的microRNA-203（miR-203）已被发现在不同癌症类型中下调或上调。本研究的目的是探讨miR-203的表达增加是否可用作上皮性卵巢癌（EOC）的非侵入性诊断和预后生物标志物。采用实时荧光定量PCR方法检测卵巢癌组织中miR-203的表达水平。miR-203在EOC组织中的表达水平显著高于邻近的非癌组织（p < 0.001）。miR-203在卵巢上皮性癌中的高表达率为65.38%（102/156）。此外，发现高miR-203表达与晚期FIGO分期（p < 0.001）、较高的组织学分级（p = 0.02）、淋巴结受累（p < 0.001）和阳性复发（p < 0.001）密切相关。此外，高miR-203表达与EOC患者的较短总生存期（p < 0.001）和较短无进展生存期（p < 0.001）相关。此外，多变量分析显示miR-203表达状态是EOC总生存期和无进展生存期的独立预测因子。这些发现首次提供了令人信服的证据，miR-203的上调可能作为一种新的分子标志物来预测EOC患者的侵袭性肿瘤进展和不良预后。
MicroRNA-203 (miR-203), possessing tumor suppressive or promotive activities, has been found to be downregulated or upregulated in different cancer types. The purpose of this study was to investigate whether the increased expression of miR-203 can be used as a noninvasive diagnostic and prognostic biomarker in epithelial ovarian cancer (EOC). Real-time quantitative PCR was performed to detect the expression levels of miR-203 in EOC tissues. The expression levels of miR-203 were significantly higher in EOC tissues compared to adjacent non-cancerous tissues (p < 0.001). High expression of miR-203 was observed in 65.38 % (102/156) of EOC. In addition, high miR-203 expression was found to be closely correlated with advanced FIGO stage (p < 0.001), higher histological grade (p = 0.02), lymph node involvement (p < 0.001), and positive recurrence (p < 0.001). Moreover, high miR-203 expression was correlated with shorter overall survival (p < 0.001) and shorter progression-free survival (p < 0.001) of EOC patients. Furthermore, multivariate analysis showed that the status of miR-203 expression was an independent predictor for both overall survival and progression-free survival in EOC. These findings provide the convincing evidence for the first time that the upregulation of miR-203 may serve as a novel molecular marker to predict the aggressive tumor progression and unfavorable prognosis of EOC patients.