Application of terbium sensitized fluorescence for the determination of fluoroquinolone antibiotics pefloxacin, ciprofloxacin and norfloxacin in serum.
Application of terbium sensitized fluorescence for the determination of fluoroquinolone antibiotics pefloxacin, ciprofloxacin and norfloxacin in serum.
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DOI：
10.1016/s0731-7085(96)02041-9
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发表时间：
1997-08
影响因子：
3.4
通讯作者：
C. Veiopoulou;P. C. Ioannou;E. Lianidou
中科院分区：
文献类型：
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作者：
C. Veiopoulou;P. C. Ioannou;E. Lianidou
建立了一种简便、快速、灵敏的氟喹诺酮类抗生素诺氟沙星（NOR）、环丙沙星（CIP）和培氟沙星（PEF）的荧光分光光度测定方法。该方法是基于在弱酸性（pH 5.5）的氯化十六烷基吡啶（CPCI）胶束溶液中，三正辛基氧化膦（TOPO）存在下，氟喹诺酮类化合物与铽离子（Tb 3+）的辐射能量转移。研究了氟喹诺酮-Tb ~（3+）-TOPO三元配合物形成的最佳条件。在优化条件下，NOR、CIP和PEF的检测限分别为1.7、1.2和4.4 nM，而所有三种药物的应用范围为0.05-10 μM。该方法已成功地应用于血清样品中的NOR，CIP和PEF的测定后，用乙腈（血清-乙腈; 1：2，v/v）脱蛋白。血清样品加标NOR、CIP和PEF（5.0-50.0 μM）的平均回收率分别为（90.3 ± 4.9）%、（105.0 ± 3.6）%和（95.3 ± 1.5）%。5.0、25.0和50.0 μM每种氟喹诺酮的运行内和日间sr值分别为1.7%-5.4%和3.3%-12.8%。本文研究了几种常用药物合用时对血清中氟喹诺酮类药物测定的影响。
A simple, rapid and sensitive spectrofluorimetric method for the determination of fluoroquinolone antibiotics, norfloxacin (NOR), ciprofloxacin (CIP) and pefloxacin (PEF) is described. The method is based on the radiative energy transfer from fluoroquinolones to terbium ions (Tb3+) in the presence of tri-n-octylphosphine oxide (TOPO) in weakly acidic (pH 5.5) micellar solution of cetylpyridinium chloride (CPCI). Optimum conditions for the formation of the fluoroquinolone-Tb3+-TOPO ternary complexes have been investigated. Under optimized conditions the detection limits are 1.7, 1.2 and 4.4 nM for NOR, CIP and PEF, respectively, while the range of application for all three drugs is 0.05–10 μM. The method has been successfully applied to the determination of NOR, CIP and PEF in serum samples after deproteinization with acetonitrile (serum-acetonitrile; 1:2, v/v). The mean recoveries from serum samples spiked with NOR, CIP and PEF (5.0–50.0 μM) were (90.3 ± 4.9), (105.0 ± 3.6) and (95.3 ± 1.5)%, respectively. Within-run and day-to-day srvalues for 5.0, 25.0 and 50.0 μM of each fluoroquinolone varied from 1.7 to 5.4% and from 3.3 to 12.8%, respectively. The influence of several usually coadministered drugs on the determination of fluoroquinolones in serum has been investigated.