Spatial distribution and species composition of prosthecate bacteria in Lake Baikal
Spatial distribution and species composition of prosthecate bacteria in Lake Baikal
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贝加尔湖人工细菌的空间分布和物种组成
DOI：
10.1134/s0026261707040145
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发表时间：
2007
期刊：
影响因子：
1.5
通讯作者：
V. Parfenova
中科院分区：
文献类型：
--
作者：
N. A. Lapteva;N. L. Bel'kova;V. Parfenova
从贝加尔湖水体中分离出了几株芽孢杆菌属和芽孢单胞菌属的检出菌。本文介绍了它们的分离和细胞数测定方法；揭示了这些微生物在湖泊中的发生频率和空间分布。分别采用传统微生物学和分子微生物学方法对可培养和不可培养的检出菌进行了种类组成鉴定。对这些不可培养细菌的DNA序列进行了系统发育比较分析，结果表明这些细菌与变形菌α亚纲的成员具有同源性。结果表明，除了具有代表性的类弧菌（caulobacter vibriides）和c菌(c)外，湖泊水柱中还存在不可培养的α -变形菌，其系统发育亲缘性不确定。用传统的微生物学方法检测。
From the water column of Lake Baikal, several strains of prosthecate bacteria belonging to the generaCaulobacterandBrevundimonaswere isolated. In this article, the methods applied for their isolation and cell number determination are described; the occurrence frequency and spatial distribution of these microorganisms in the lake are demonstrated. Characterization of the species composition of cultivable and uncultivable prosthecate bacteria was carried out using the methods of traditional and molecular microbiology, respectively. A comparative phylogenetic analysis of the DNA sequences of uncultivable bacteria, which showed homology to the members of the alpha subclass of proteobacteria, was carried out. It was demonstrated that the lake water column is inhabited by uncultivable alpha-proteobacteria of uncertain phylogenetic affinity, in addition to representatives of the speciesCaulobacter vibrioidesandC. leidyi, which were detected by traditional microbiological methods.