Targeted lipidomics using electron capture atmospheric pressure chemical ionization mass spectrometry
Targeted lipidomics using electron capture atmospheric pressure chemical ionization mass spectrometry
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DOI：
10.1002/rcm.1170
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发表时间：
2003-01-01
影响因子：
2
通讯作者：
Blair, IA
中科院分区：
文献类型：
--
作者：
Lee, SH;Williams, MV;Blair, IA
人们越来越需要能够进行定量脂质组学分析作为蛋白质组学研究的补充。使用基于电喷雾电离 (ESI) 或大气压化学电离 (APCI) 结合液相色谱/串联质谱 (LC/MS/MS) 的方法可以获得蛋白质组学分析的最高特异性。对于脂质组学分析，通常需要能够分离对映异构体和区域异构体。当使用基于传统反相色谱的方法时，这可能非常具有挑战性。使用手性柱的正相色谱可以显着提高对映异构体和区域异构体的分辨率。然而，传统的 ESI-和 APCI-MS/MS 的灵敏度有限，这使得在仅存在微量非酯化生物活性脂质的细胞培养系统中进行研究变得困难。电子捕获 APCI-MS/MS 的使用克服了这个问题。可以使用稳定同位素稀释方法结合正相手性色谱和电子捕获 APCI-MS/MS 来定量不同生物活性脂质的对映异构体和区域异构体。该方法允许描绘培养物中维持的大鼠上皮细胞的脂质组学概况，并允许评估非选择性脂氧合酶抑制剂的效果。版权所有 (C) 2003 John Wiley Sons, Ltd.
There is an increasing need to be able to conduct quantitative lipidomics analyses as a complement to proteomics studies. The highest specificity for proteomics analysis can be obtained using methodology based on electrospray ionization (ESI) or atmospheric pressure chemical ionization (APCI) coupled with liquid chromatography/tandem mass spectrometry (LC/MS/MS). For lipidomics analysis it is often necessary to be able to separate enantiomers and regioisomers. This can be very challenging when using methodology based on conventional reversed-phase chromatography. Normal-phase chromatography using chiral columns can provide dramatic improvements in the resolution of enantiomers and regioisomers. However, conventional ESI- and APCI-MS/MS has limited sensitivity, which makes it difficult to conduct studies in cell culture systems where only trace amounts of non-esterified bioactive lipids are present. The use of electron capture APCI-MS/MS overcomes this problem. Enantiomers and regioisomers of diverse bioactive lipids can be quantified using stable isotope dilution methodology coupled with normal-phase chiral chromatography and electron capture APCI-MS/MS. This methodology has allowed a lipidomics profile from rat epithelial cells maintained in culture to be delineated and allowed the effect of a non-selective lipoxygenase inhibitor to be assessed. Copyright (C) 2003 John Wiley Sons, Ltd.