Expression of EBV-encoded oncogenes and EBV-like virions in multiple canine tumors
Expression of EBV-encoded oncogenes and EBV-like virions in multiple canine tumors
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DOI：
10.1016/j.vetmic.2012.12.022
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发表时间：
2013-04-12
影响因子：
3.3
通讯作者：
Sun, H. Sunny
中科院分区：
文献类型：
--
作者：
Chiu, Hung-Chuan;Chow, Kuan-Chih;Sun, H. Sunny
EB病毒（Epstein-Barr virus，EBV）是一种普遍存在的人类肿瘤病毒。我们和其他人之前的研究表明，宠物狗经常遇到EB病毒或EB病毒相关病毒感染。在这项研究中，我们探讨了EBV是否参与犬的恶性肿瘤。应用聚合酶链反应（PCR）技术对12例犬肿瘤标本中的10例（包括8例口腔肿瘤）进行了EBV特异性BamH Ⅰ W序列的检测。用逆转录PCR方法检测了12例大肠癌中8例和7例潜伏膜蛋白1（LMP 1）和BamH Ⅰ H Ⅰ阅读框1（BHRF 1）基因的表达。一种新的LMP1变体T0905在5个犬肿瘤标本中占主导地位，并发现存在于EBV阳性的人BC-2细胞中。另一种LMP1变体T0902与人类肿瘤变体JB7相似。从这些犬肿瘤中鉴定的BHRF 1序列与B95 - 8病毒株的序列相同。LMP1蛋白和EB病毒编码的RNA（EBER）分别用免疫组化和荧光原位杂交方法在几种肿瘤中检测到，特别是在口腔无黑色素性黑色素瘤的肿瘤巢中。此外，EBV样病毒粒子采用疱疹病毒的出口途径检测在眦成纤维细胞骨肉瘤和口腔无色素性melanotic. In结论，我们报告的表达BHRF1转录（病毒抗凋亡蛋白），LMP1（病毒癌蛋白）转录和蛋白，EBER（病毒致癌RNA），和EBV样病毒粒子在多种犬肿瘤。BHRF1的身份和犬和人肿瘤之间LMP1变异的相似性表明犬和人EBV之间存在密切的进化关系，或者存在人畜共患传播的可能性。(C)2013爱思唯尔有限公司版权所有。
Epstein-Barr virus (EBV) is a ubiquitous human oncovirus. Previous studies by us and others have indicated that pet dogs frequently encounter EBV or EBV-related viral infection. In this study, we explored whether EBV is involved in canine malignancies in dogs. EBV-specific BamHI W sequence was detected by polymerase chain reaction (PCR) in 10 of 12 canine tumor specimens, including 8 of 10 oral tumors. Using reverse transcription-PCR, gene expressions of latent membrane protein 1 (LMP 1) and BamHI H rightward reading frame 1 (BHRF1) were identified in 8 and 7 of 12 specimens, respectively. A novel LMP1 variant, T0905, was predominant in 5 canine tumor specimens and found to exist in EBV positive human BC-2 cells. Another LMP1 variant, T0902, was similar to human tumor variant JB7. The BHRF1 sequence identified from these canine tumors was identical to that of the B95-8 viral strain. LMP1 protein and EBV-encoded RNA (EBER) were detected by immunohistochemistiy and fluorescent in situ hybridization, respectively, in several tumors, particularly in tumor nests of oral amelanotic melanomas. Furthermore, EBV-like virions adopting a herpesvirus egress pathway were detected in a canthal fibroblastic osteosarcoma and an oral amelanotic melanoma.In conclusion, we report the expressions of BHRF1 transcript (a viral anti-apoptotic protein), LMP1 (a viral oncoprotein) transcript and protein, EBER (a viral oncogenic RNA), and EBV-like virions in multiple canine tumors. The identity of BHRF1 and the resemblance of LMP1 variants between canine and human tumors indicate either a close evolutionary relationship between canine and human EBV, or the possibility of zoonotic transmission. (C) 2013 Elsevier B.V. All rights reserved.