lncRNA FEZF1-AS1 Is Associated With Prognosis in Lung Adenocarcinoma and Promotes Cell Proliferation, Migration, and Invasion.
lncRNA FEZF1-AS1 Is Associated With Prognosis in Lung Adenocarcinoma and Promotes Cell Proliferation, Migration, and Invasion.
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DOI：
10.3727/096504018x15199482824130
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发表时间：
2018-12-27
影响因子：
3.1
通讯作者：
Lu S
中科院分区：
文献类型：
--
作者：
Liu Z;Zhao P;Han Y;Lu S
据报道，长非编码 RNA (lncRNA) 在肿瘤发生中发挥重要作用。在本研究中，我们证明lncRNA前脑胚胎锌指蛋白1（FEZF1）反义RNA1（FEZF1-AS1）在人肺腺癌（LAD）组织和细胞系中显着上调，并且与不良预后相关。功能丧失表明，FEZF1-AS1 表达的缺失显着抑制了 LAD 细胞的增殖、侵袭和迁移。进一步的研究表明，FEZF1-AS1 的下调会降低 LAD 细胞中其正义同源基因 FEZF1 的 mRNA 和蛋白质表达，反之亦然。相关分析表明,LAD组织中FEZF1-AS1与FEZF1表达量呈正相关。此外，救援实验证实了 FEZF1-AS1 在 LAD 中的功能是由 FEZF1 介导的。我们的研究结果表明，FEZF1-AS1 的失调会导致 LAD 的进展，这可能是 LAD 治疗的潜在靶点。
Long noncoding RNAs (lncRNAs) have been reported to play important roles in tumorigenesis. In the present study, we demonstrated that lncRNA forebrain embryonic zinc finger protein 1 (FEZF1) antisense RNA1 (FEZF1-AS1) is markedly upregulated in human lung adenocarcinoma (LAD) tissues and cell lines and is associated with poor prognosis. Loss of function revealed that deletion of FEZF1-AS1 expression significantly inhibited the LAD cell proliferation, invasion, and migration. Further studies revealed that downregulation of FEZF1-AS1 reduced mRNA and protein expression of its sense-cognate gene FEZF1 in LAD cells, and vice versa. Correlation analysis indicated that there was a positive correlation between FEZF1-AS1 and FEZF1 expression in LAD tissues. Additionally, rescue assay confirmed that the function of FEZF1-AS1 in LAD was mediated by FEZF1. Our findings suggested that dysregulation of FEZF1-AS1 contributed to the progression of LAD, which might be a potential target for LAD therapy.