Molecular Cloning of Rat Spetex2 Family Genes Mapped on Chromosome 15p16, Encoding a 23-Kilodalton Protein Associated with the Plasma Membranes of Haploid Spermatids1
Molecular Cloning of Rat Spetex2 Family Genes Mapped on Chromosome 15p16, Encoding a 23-Kilodalton Protein Associated with the Plasma Membranes of Haploid Spermatids1
复制标题
定位于染色体 15p16 的大鼠 Spetex2 家族基因的分子克隆，编码与单倍体精子细胞质膜相关的 23 千道尔顿蛋白
作者：
Y. Iwamoto;T. Kaneko;Junko Ichinose;Takayuki Mōri;Y. Shibata;K. Toshimori;H. Iida
摘要我们利用差异显示技术结合cDNA克隆技术，从大鼠中分离到一个新基因Spetex 2，该基因的开放阅读框为582个核苷酸，编码194个氨基酸。Spetex 2 mRNA在大鼠睾丸和脾脏中高表达，且在大鼠睾丸中的表达随发育而上调。原位杂交显示Spetex 2 mRNA主要表达于生精上皮内的1-13步的单倍体精子细胞中。对美国国家生物技术信息中心大鼠基因组数据库的BLAST搜索显示，Spetex 2基因由4个外显子组成，并定位于大鼠染色体15 p16上的一个簇中的至少18个位点，表明这些基因在基因组DNA的长片段上以重复串联阵列的形式存在。通过共聚焦激光扫描显微镜的免疫细胞化学分析，SPETEX 2蛋白被检测为在单倍体精子细胞的细胞外周上的点状分布（步骤1-13），但在其他生精细胞中未观察到。基于这些数据，我们推测SPETEX 2可能与大鼠睾丸精子细胞的分化有关。
Abstract We used differential display in combination with cDNA cloning to isolate a novel rat gene, designated as Spetex2, that has an open reading frame of 582 nucleotides, encoding a protein of 194 amino acids. Spetex2 mRNA was highly expressed in testis and spleen, and its expression in rat testis was developmentally up-regulated. In situ hybridization revealed that Spetex2 mRNA was predominantly expressed in haploid spermatids at steps 1–13 within the seminiferous epithelium. A BLAST search against rat genome databases at the National Center for Biotechnology Information revealed that the Spetex2 gene is composed of four exons and is mapped to at least 18 loci in a cluster on rat chromosome 15p16, indicating that the genes occur as a repeated tandem array over a long stretch of genomic DNA. By immunocytochemical analysis with confocal laser-scanning microscopy, SPETEX2 protein was detected as a dot-like distribution on the cell periphery of haploid spermatids (steps 1–13) but was not observed in other spermatogenic cells. On the basis of these data, we hypothesize that SPETEX2 might be correlated with cell differentiation of spermaytids in rat testis.
影响因子：
2.7
作者：
Shinohara, T;Avarbock, MR;Brinster, RL
通讯作者：
Brinster, RL