Immune responses induced by lower airway mucosal immunisation with a human papillomavirus type 16 virus-like particle vaccine
Immune responses induced by lower airway mucosal immunisation with a human papillomavirus type 16 virus-like particle vaccine
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DOI：
10.1016/j.vaccine.2005.02.019
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发表时间：
2005-05-25
期刊：
影响因子：
5.5
通讯作者：
De Grandi, P
中科院分区：
文献类型：
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作者：
Nardelli-Haefliger, D;Lurati, F;De Grandi, P
宫颈癌是由人乳头瘤病毒 (HPV) 宫颈感染引起的。尤其是HPV16。先前的研究表明，女性肌肉注射 HPV16 病毒样颗粒 (VLP) 疫苗可诱导强烈的 IgG 反应，并防止生殖器 HPV16 感染。然而，避免肠胃外注射同时诱导粘膜免疫的替代给药途径可能有助于在某些情况下实施疫苗，并部分克服排卵妇女宫颈中 HPV16 抗体的显着变化。在这项研究中，女性通过鼻雾化、支气管雾化或肌内和气雾剂疫苗接种相结合的方式接种了逐渐增加剂量的 HPV16LI VLP。替代疫苗接种途径的耐受性良好，许多接受气雾剂疫苗接种的志愿者表现出的血清抗体滴度与肌肉注射疫苗诱导的抗体滴度相当。通过气雾剂疫苗接种诱导粘膜免疫应答，如通过诱导PBMC中的抗HPV16 VLP IgA分泌细胞和分泌物中的SIgA来证明。我们的数据表明，HPV VLP 的气雾给药可能是肠外注射的潜在替代方案。 (c) 2005 Elsevier Ltd. 保留所有权利。
Cervical cancer results from cervical infection by human papillomaviruses (HPV). especially HPV16. Previous studies have shown that intramuscular vaccination of women with an HPV16 virus-like particle (VLP) vaccine induced a strong IgG response and protected against genital HPV16 infection. However, an alternative route of administration that avoids parenteral injection while inducing mucosal immunity might facilitate vaccine implementation in some settings, and partially overcome the substantial variation in HPV16 antibodies at the cervix seen in ovulating women. In this study, women were vaccinated with escalating doses of HPV16LI VLPs Via nasal nebulisation, bronchial aerosolisation, or a combination of intramuscular and aerosol vaccination. The alternative routes of vaccination were well tolerated and many of the volunteers who received aerosol vaccinations exhibited serum antibody titers that were comparable to those induced by intramuscular vaccination. A mucosal immune response was induced by aerosol vaccination as demonstrated by the induction of anti-HPV16 VLP IgA secreting cells in PBMC and SIgA in secretions. Our data suggest that aerosol administration of HPV VLPs may represent a potential alternative to parenteral injection. (c) 2005 Elsevier Ltd. All rights reserved.