Identification of Peach NAP Transcription Factor Genes and Characterization of their Expression in Vegetative and Reproductive Organs during Development and Senescence.
Identification of Peach NAP Transcription Factor Genes and Characterization of their Expression in Vegetative and Reproductive Organs during Development and Senescence.
复制标题
桃 NAP 转录因子基因的鉴定及其在发育和衰老过程中营养和生殖器官中的表达特征
DOI：
10.3389/fpls.2016.00147
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发表时间：
2016
影响因子：
5.6
通讯作者：
Zhao C
中科院分区：
文献类型：
--
作者：
Li F;Li J;Qian M;Han M;Cao L;Liu H;Zhang D;Zhao C
NAP（NAC样，由AP 3/P1激活）转录因子属于NAC转录因子家族的一个亚家族，被认为在调节植物生长发育中具有重要作用。然而，在蔷薇科植物中关于该亚科的信息很少。我们在桃基因组中鉴定了7个NAP基因。PpNAP 2属于NAP I组，含有一个保守的转录激活区。其他PpNAP基因属于NAP II组。PpNAP基因在不同器官和发育阶段的表达模式不同。PpNAP 1和PpNAP 2在成熟和衰老的花中高表达，而在叶片、果实和花蕾中不表达。PpNAP 3和PpNAP 5仅在叶片中表达。PpNAP 4在成熟和衰老的果实中表达量较高，而PpNAP 6和PpNAP 7在成熟和衰老的叶片和花中表达上调。在果实发育过程中，PpNAP 4和PpNAP 6的表达量在S1和S4期迅速增加，表明这两个基因与第一指数生长期和果实成熟有关。在果实成熟软化期，PpNAP 1，PpNAP 4和PpNAP 6的表达水平高，在贮藏初期，这是伴随着乙烯产生的快速增加。PpNAP 1、PpNAP 4和PpNAP 6的表达在贮藏中后期缓慢增加，在贮藏末期达到高峰。此外，脱落酸（阿坝）处理的水果更软，产生更多的乙烯比对照。此外，PpNAP 1，PpNAP 4和PpNAP 6的表达水平较高的ABA处理的水果。这些结果表明，PpNAP 1，PpNAP 4和PpNAP 6是响应阿坝，并可能调节桃果实成熟。
The NAP (NAC-like, activated by AP3/P1) transcription factor belongs to a subfamily of the NAC transcription factor family, and is believed to have an important role in regulating plant growth and development. However, there is very little information about this subfamily in Rosaceous plants. We identified seven NAP genes in the peach genome. PpNAP2 was categorized in the NAP I group, and contained a conserved transcription activation region. The other PpNAP genes belonged to the NAP II group. The expression patterns of the PpNAP genes differed in various organs and developmental stages. PpNAP1 and PpNAP2 were highly expressed in mature and senescing flowers, but not in leaves, fruits, and flower buds. PpNAP3 and PpNAP5 were only expressed in leaves. The PpNAP4 expression level was high in mature and senescing fruits, while PpNAP6 and PpNAP7 expression was up-regulated in mature and senescent leaves and flowers. During the fruit development period, the PpNAP4 and PpNAP6 expression levels rapidly increased during the S1 and S4 stages, which suggests these genes are involved in the first exponential growth phase and fruit ripening. During the fruit ripening and softening period, the PpNAP1, PpNAP4, and PpNAP6 expression levels were high during the early storage period, which was accompanied by a rapid increase in ethylene production. PpNAP1, PpNAP4, and PpNAP6 expression slowly increased during the middle or late storage periods, and peaked at the end of the storage period. Additionally, abscisic acid (ABA)-treated fruits were softer and produced more ethylene than the controls. Furthermore, the PpNAP1, PpNAP4, and PpNAP6 expression levels were higher in ABA-treated fruits. These results suggest that PpNAP1, PpNAP4, and PpNAP6 are responsive to ABA and may regulate peach fruit ripening.