Connective tissue cells, but not muscle cells, are involved in establishing the proximo-distal outcome of limb regeneration in the axolotl
Connective tissue cells, but not muscle cells, are involved in establishing the proximo-distal outcome of limb regeneration in the axolotl
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DOI：
10.1242/dev.081752
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发表时间：
2013-02-01
期刊：
影响因子：
4.6
通讯作者：
Tanaka, Elly M.
中科院分区：
文献类型：
--
作者：
Nacu, Eugen;Glausch, Mareen;Tanaka, Elly M.
在蝾螈肢体再生过程中，只有离截肢平面较远的结构才能再生，这一特性被称为远端转化规则。多种细胞类型参与肢体再生;因此，确定哪些细胞类型参与远端转化对于理解再生的近端-远端结果是如何实现的是重要的。我们发现，结缔组织来源的芽基细胞服从远端转化的规则。他们也有核MEIS，它可以作为上臂身份调节器，只有在上臂截肢。相比之下，肌源性细胞不遵守远端转化的规则，并且在任何近端远端水平截肢后显示核MEIS。这些结果表明，结缔组织细胞，而不是肌源性细胞，参与建立近端再生的结果，并可能指导肌肉图案。此外，我们表明，类似于肢体发育，肌肉再生模式的影响β-连环蛋白信号。
During salamander limb regeneration, only the structures distal to the amputation plane are regenerated, a property known as the rule of distal transformation. Multiple cell types are involved in limb regeneration; therefore, determining which cell types participate in distal transformation is important for understanding how the proximo-distal outcome of regeneration is achieved. We show that connective tissue-derived blastema cells obey the rule of distal transformation. They also have nuclear MEIS, which can act as an upper arm identity regulator, only upon upper arm amputation. By contrast, myogenic cells do not obey the rule of distal transformation and display nuclear MEIS upon amputation at any proximo-distal level. These results indicate that connective tissue cells, but not myogenic cells, are involved in establishing the proximo-distal outcome of regeneration and are likely to guide muscle patterning. Moreover, we show that, similarly to limb development, muscle patterning in regeneration is influenced by beta-catenin signalling.