Correlation between pathology and neuromelanin MR imaging in Parkinson's disease and dementia with Lewy bodies
Correlation between pathology and neuromelanin MR imaging in Parkinson's disease and dementia with Lewy bodies
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DOI：
10.1007/s00234-013-1199-9
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发表时间：
2013-08-01
期刊：
影响因子：
2.8
通讯作者：
Ogawa, Toshihide
中科院分区：
文献类型：
--
作者：
Kitao, Shinichiro;Matsusue, Eiji;Ogawa, Toshihide
为探讨正常人、帕金森病（PD）和路易体痴呆（DLB）患者黑质神经黑素（NmMRI）信号改变的病理背景，对3例经10%福尔马林固定的尸检中脑进行了NmMRI检查（正常对照、DLB和PD）与3T成像系统，使用具有磁化转移对比脉冲的3D梯度回波T1加权序列。进行中脑的神经病理学检查，并测定神经黑素阳性神经元的密度（每平方毫米的数量）。铁沉积在中脑的程度也进行了评价，使用铁蛋白免疫组织化学。此外，我们直接相关的对比信号比在SNc和神经黑素的神经元的密度。弥漫性高信号区在SNc反映了保存完好的神经黑素的神经元在正常对照组的情况下，而在SNc的等信号区显示严重损失的神经黑素的神经元在DLB和PD的情况下。SNc中信号强度的增加显然不受铁沉积的影响。此外，信号强度和神经黑素神经元的密度之间的显着正相关性被视为在SNc。基于直接相关性之间的神经mMRI和神经病理结果，信号强度在SNc是密切相关的神经黑素神经元的数量，但不受铁沉积。
Direct correlation between neuropathological findings and postmortem neuromelanin MR imaging (NmMRI) was performed in the substantia nigra pars compacta (SNc) to clarify the pathological background of the signal changes in normal, Parkinson's disease (PD), and dementia with Lewy bodies (DLB) cases.NmMRI of 10 % formalin-fixed autopsied midbrains was performed in three cases (normal control, DLB, and PD) with a 3T imaging system, using a 3D gradient echo T1-weighted sequence with a magnetization transfer contrast pulse. Neuropathological examinations of the midbrains were performed, and the density of neuromelanin-positive neurons (number per square millimeter) was determined. The extent of iron deposition in the midbrain was also evaluated using ferritin immunohistochemistry. Furthermore, we directly correlated the contrast signal ratio in the SNc and the density of neuromelanin-containing neurons.Diffuse hyperintense areas in the SNc reflected well-preserved neuromelanin-containing neurons in the normal control case, whereas an iso-intense area in the SNc showed severe loss of neuromelanin-containing neurons in the DLB and PD cases. Increased signal intensity in the SNc was apparently not influenced by iron deposition. Furthermore, a significant positive correlation between signal intensity and the density of neuromelanin-containing neurons was seen in the SNc.Based on the direct correlation between postportem NmMRI and neuropathological findings, signal intensity in the SNc is closely related to the quantity of neuromelanin-containing neurons but is not influenced by iron deposition.