Mapping of quantitative trait loci controlling partial resistance against rust incited by Uromyces pisi (Pers.) Wint. in a Pisum fulvum L. intraspecific cross
Mapping of quantitative trait loci controlling partial resistance against rust incited by Uromyces pisi (Pers.) Wint. in a Pisum fulvum L. intraspecific cross
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DOI：
10.1007/s10681-010-0141-z
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发表时间：
2010-09-01
期刊：
影响因子：
1.9
通讯作者：
Torres, A. M.
中科院分区：
文献类型：
--
作者：
Barilli, E.;Satovic, Z.;Torres, A. M.
利用QTL分析技术，对豌豆锈菌（Uromycespisi（E.g.）温特，在两个野生豌豆（PisumfulvumL.）材料，IFPI 3260（抗）和IFPI 3251（感）。亲本系和所有分离群体都显示出完全相容的相互作用（高感染类型），这表明不存在过敏反应。然而，观察到整个植物的症状面积百分比（疾病严重程度）的差异。构建的遗传图谱覆盖1283.3cM，包括146个标记（144个随机扩增多态性DNA（RAPD）标记和2个序列标记位点（STS）标记），分布在9个连锁群中。解释总表型变异的63%的QTL位于连锁群3。位于该QTL侧翼的RAPD标记（OPY11（1316）和OPV17（1078））在SCARs中转化后，应允许进行可靠的标记辅助选择以获得抗锈性。
A quantitative trait loci (QTL) associated with resistance to pea rust, caused by the fungus Uromyces pisi (Pers.) Wint., has been identified in a F(2) population derived from an intraspecific cross between two wild pea (Pisum fulvum L.) accessions, IFPI3260 (resistant) and IFPI3251 (susceptible). Both parental lines and all the segregating population displayed a fully compatible interaction (high infection type), which indicates absence of hypersensitive response. Nevertheless, differences on the percentage of symptomatic area of the whole plant (disease severity) were observed. A genetic map was developed covering 1283.3 cM and including 146 markers (144 random amplified polymorphic DNA (RAPDs) and two sequence tagged sites (STSs) markers) distributed in 9 linkage groups. A QTL explaining 63% of the total phenotypic variation was located in linkage group 3. RAPDs markers (OPY11(1316) and OPV17(1078)) flanking this QTL should allow, after their conversion in SCARs, a reliable marker-assisted selection for rust resistance.