Highly Excretable Gold Supraclusters for Translatable In Vivo Raman Imaging of Tumors.
Highly Excretable Gold Supraclusters for Translatable In Vivo Raman Imaging of Tumors.
复制标题
用于肿瘤体内可转化拉曼成像的高度排泄金超簇。
DOI：
10.1021/acsnano.2c10378
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发表时间：
2023
期刊：
影响因子：
17.1
通讯作者：
Rao,Jianghong
中科院分区：
文献类型：
--
作者：
Yu,JungHo;Jeong,MyeongSeon;Cruz,EmmaOlivia;Alam,IsratS;Tumbale,SpencerK;Zlitni,Aimen;Lee,SongYeul;Park,YongIl;Ferrara,Katherine;Kwon,Seung-Hae;Gambhir,SanjivS;Rao,Jianghong
拉曼光谱通过读出类似指纹的光谱，为活体临床成像提供了极好的特异性。为了在活体拉曼成像中获得足够的灵敏度，使用大于10 nm的金属金纳米颗粒通过表面增强拉曼散射(SERS)放大拉曼信号。然而，无法排出如此大的金纳米颗粒限制了拉曼成像的翻译。在这里，我们介绍了拉曼活性金属金超光泽，是可生物降解和排泄的纳米团簇。虽然金纳米团簇的小尺寸削弱了电磁场增强效应，但由于近红外共振拉曼染料的高负载量和金属超聚体对荧光背景的抑制，超聚体表现出与大尺寸金纳米颗粒SERS纳米标记相当的明亮和显著的拉曼散射。超强光的拉曼散射是pH响应的，我们成功地对小鼠酸性肿瘤进行了活体拉曼成像。此外，与在肝脏和脾中停留超过4个月的大的金纳米颗粒相比，超光泽解离成小的纳米簇，在同一时期，给药小鼠的73%的剂量被排出。这里展示的高度可排泄的拉曼超滤液为活体拉曼成像的临床应用提供了巨大的潜力。
Raman spectroscopy provides excellent specificity forin vivopreclinical imaging through a readout of fingerprint-like spectra. To achieve sufficient sensitivity forin vivoRaman imaging, metallic gold nanoparticles larger than 10 nm were employed to amplify Raman signals via surface-enhanced Raman scattering (SERS). However, the inability to excrete such large gold nanoparticles has restricted the translation of Raman imaging. Here we present Raman-active metallic gold supraclusters that are biodegradable and excretable as nanoclusters. Although the small size of the gold nanocluster building blocks compromises the electromagnetic field enhancement effect, the supraclusters exhibit bright and prominent Raman scattering comparable to that of large gold nanoparticle-based SERS nanotags due to high loading of NIR-resonant Raman dyes and much suppressed fluorescence background by metallic supraclusters. The bright Raman scattering of the supraclusters was pH-responsive, and we successfully performedin vivoRaman imaging of acidic tumors in mice. Furthermore, in contrast to large gold nanoparticles that remain in the liver and spleen over 4 months, the supraclusters dissociated into small nanoclusters, and 73% of the administered dose to mice was excreted during the same period. The highly excretable Raman supraclusters demonstrated here offer great potential for clinical applications ofin vivoRaman imaging.