Morphological and cytoskeletal alterations of nervous system tumor cells with different culturing methods
Morphological and cytoskeletal alterations of nervous system tumor cells with different culturing methods
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DOI：
10.3892/ijo.2011.945
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发表时间：
2011-05-01
影响因子：
5.2
通讯作者：
Naito, Zenya
中科院分区：
文献类型：
--
作者：
Matsuda, Yoko;Kawamoto, Yoko;Naito, Zenya
细胞培养是分子和细胞生物学中最重要的研究方法之一，并且已经开发了各种培养系统，包括二维（2D）、三维（3D）和漂浮培养系统。在本研究中，我们检查了在 2D、3D 和浮动细胞培养物中生长的三种不同类型的神经系统肿瘤细胞的形态变化和细胞骨架蛋白的不同表达模式。 A172、KG-1-C 和 IMR-32 细胞表现出明显的形态变化，具体取决于细胞培养方法。在 2D 和 313 培养物中，在最靠近板表面的细胞水平上清楚地观察到 F-肌动蛋白表达。另一方面，F-肌动蛋白的表达在漂浮培养系统中较弱。在 2D 培养中，α-微管蛋白在细胞的细胞质中检测到，但在浮动和 3D 培养中，α-微管蛋白在球体和球体的外围区域表达。总之，这项研究表明，神经系统肿瘤细胞根据培养方法的不同，表现出不同的形态变化和不同的细胞骨架蛋白表达模式。
Cell culture is one of the most important methods of research :in molecular and cellular biology, and various culture systems have been developed, including two-dimensional (2D), three-dimensional (3D) and floating culture systems. In the present study, we examined morphological changes and different expression patterns of cytoskeletal proteins in three different types of nervous system tumor cells grown in 2D, 3D and floating cell cultures. A172, KG-1-C and IMR-32 cells showed marked morphological changes, depending on the cell culture methods. F-actin expression was clearly observed at the level of the cells nearest the plate surface in 2D and 313 cultures. On the other hand, expression of F-actin was weak in the floating culture system. a-tubulin was detected in the cytoplasm of cells in 2D culture, but in floating and 3D cultures, a-tubulin was expressed in the peripheral regions of spheres and spheroids. In conclusion, this study demonstrated that nervous system tumor cells showed different alterations in morphology, and different cytoskeletal protein expression patterns, depending on the culture methods.