Characterization of non-Hodgkin's lymphomas using multiple cell markers. Immunologic, morphologic, and cytochemical studies of 72 cases.
Characterization of non-Hodgkin's lymphomas using multiple cell markers. Immunologic, morphologic, and cytochemical studies of 72 cases.
复制标题
使用多种细胞标记物表征非霍奇金淋巴瘤。
作者：
G. Pinkus;J. Said
采用多种技术对72例（87例标本）各种非霍奇金淋巴瘤的组织进行细胞标记物分析。评价细胞悬液中的E、EAC和IgGEA玫瑰花结形成细胞; Fc受体细胞;和表面免疫球蛋白携带细胞。冷冻切片研究拓扑定义的EAC结合细胞。细胞化学测定和免疫过氧化物酶法检测细胞内免疫球蛋白和溶菌酶补充其他技术在评估浸润含有大的肿瘤细胞。B细胞恶性肿瘤包括该系列的58例（80%），包括高分化和中分化淋巴细胞性淋巴瘤（10/10）;结节性（23/23）和弥漫性（10/18）低分化淋巴细胞性淋巴瘤;以及混合淋巴细胞性淋巴瘤-“组织细胞性”（3/3）、“未分化”（3/3）和“组织细胞性”（9/13）类型。结节性淋巴瘤的特征是B细胞肿瘤，但也显示了一个突出的T淋巴细胞群体（39 +/- 12%）。碱性磷酸酶活性是滤泡袖淋巴样细胞的细胞化学标志物，在中等分化淋巴细胞型B细胞淋巴瘤中观察到最一致（4/6例）。在一些弥漫性淋巴瘤中，冷冻切片研究（EAC切片）提示预先存在结节性增生。一个不寻常的大细胞型B细胞淋巴瘤表现出IgGEA玫瑰花结形成和IgG的Fc部分的强受体。10例淋巴瘤（14%）为T细胞型，包括弥漫性低分化淋巴细胞淋巴瘤（5/18，包括3例淋巴母细胞淋巴瘤）、Sézary综合征（1例）、蕈样肉芽肿（1例）和细胞学特征性大细胞（“组织细胞”）淋巴瘤（3/13）。酸性磷酸酶活性是T细胞恶性肿瘤的一致标志物，其中一些还显示α-萘丁酸酯酶活性。未检测到真正的组织细胞淋巴瘤。3例弥漫性低分化淋巴细胞性淋巴瘤和1例组织细胞性淋巴瘤均为阴性。
Tissues from 72 cases (87 specimens) of various non-Hodgkin's lymphomas were analyzed for cell markers using multiple techniques. Cell suspensions were evaluated for E, EAC, and IgGEA rosette forming cells; Fc receptor cells; and surface immunoglobulin bearing cells. Cryostat section studies topographically defined EAC binding cells. Cytochemical determinations and immunoperoxidase methods for detection of intracellular immunoglobulin and lysozyme complemented other techniques in evaluating infiltrates containing large neoplastic cells. B-cell malignancies comprised 58 cases (80%) of this series and included well and moderately well differentiated lymphocytic lymphomas (10/10); nodular (23/23) and diffuse (10/18) poorly differentiated lymphocytic lymphomas; and lymphomas of mixed lymphocytic-"histiocytic" (3/3), "undifferentiated" (3/3), and "histiocytic" (9/13) types. Nodular lymphomas were characterized as B-cell neoplasms but also revealed a prominent population of T lymphocytes (39 +/- 12%). Alkaline phosphatase activity, a cytochemical marker for lymphoid cells of follicular cuffs, was most consistently observed in B-cell lymphomas of moderately well differentiated lymphocytic type (4/6 cases). In some diffuse lymphomas, cryostat section studies (EAC rosettes) suggested a pre-existing nodular proliferation. One unusual B-cell lymphoma of large cell type exhibited IgGEA rosette formation and a strong receptor for the Fc portion of IgG. Ten lymphomas (14%) were of T-cell type and were represented by cases of diffuse poorly differentiated lymphocytic lymphoma (5/18, including 3 lymphoblastic lymphomas), Sézary syndrome (1), mycosis fungoides (1), and a cytologically distinctive large cell ("histiocytic") lymphoma (3/13). Acid phosphatase activity was a consistent marker for the T-cell malignancies, some of which also revealed alpha-naphthyl butyrate esterase activity. No true histiocytic lymphomas were detected. Three cases of diffuse poorly differentiated lymphocytic lymphoma and one "histiocytic" lymphoma were null.