Reactivation of the paternal X chromosome in early mouse embryos
Reactivation of the paternal X chromosome in early mouse embryos
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DOI：
10.1126/science.1092674
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发表时间：
2004-01-30
期刊：
影响因子：
56.9
通讯作者：
Brockdorff, N
中科院分区：
文献类型：
--
作者：
Mak, W;Nesterova, TB;Brockdorff, N
一般认为，父系印记X染色体失活只发生在小鼠胚胎的胚外谱系中，而来自内细胞团（ICM）的胚胎细胞只经历随机的X染色体失活。在这里，我们表明，印记X失活，事实上，发生在所有细胞的早期胚胎和父亲的X，然后选择性地重新激活分配给ICM的细胞。这与更分化的细胞类型形成对比，其中X失活是高度稳定的并且通常是不可逆的。我们的观察表明，在早期发育中基因组可塑性的一个重要组成部分是逆转遗传基因沉默决定的能力。
It is generally accepted that paternally imprinted X inactivation occurs exclusively in extraembryonic lineages of mouse embryos, whereas cells of the embryo proper, derived from the inner cell mass (ICM), undergo only random X inactivation. Here we show that imprinted X inactivation, in fact, occurs in all cells of early embryos and that the paternal X is then selectively reactivated in cells allocated to the ICM. This contrasts with more differentiated cell types where X inactivation is highly stable and generally irreversible. Our observations illustrate that an important component of genome plasticity in early development is the capacity to reverse heritable gene silencing decisions.