A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells.
A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells.
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DOI：
10.1073/pnas.0508793103
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发表时间：
2006-05
影响因子：
11.1
通讯作者：
M. Sakaguchi;T. Shingo;T. Shimazaki;H. Okano;M. Shiwa;S. Ishibashi;H. Oguro;M. Ninomiya;T. Kadoya-T
中科院分区：
文献类型：
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作者：
M. Sakaguchi;T. Shingo;T. Shimazaki;H. Okano;M. Shiwa;S. Ishibashi;H. Oguro;M. Ninomiya;T. Kadoya-T
在成年哺乳动物前脑的脑室下区，神经干细胞（NSCs）驻留并增殖以产生年轻的神经元。我们筛选因素，促进神经干细胞在体外增殖的最近开发的蛋白质组学技术，蛋白质芯片系统。在这个屏幕上，我们确定了可溶性碳水化合物结合蛋白，半乳糖凝集素-1，作为候选人。我们在本文中显示，半乳糖凝集素-1在缓慢分裂的室管膜下区星形胶质细胞的子集中表达，所述星形胶质细胞包括NSC。基于脑室内灌注实验和基因敲除小鼠表型分析的结果，我们证明了半乳糖凝集素-1是一种内源性因子，可促进成体脑中神经干细胞的增殖。
In the subventricular zone of the adult mammalian forebrain, neural stem cells (NSCs) reside and proliferate to generate young neurons. We screened factors that promoted the proliferation of NSCs in vitro by a recently developed proteomics technique, the ProteinChip system. In this screen, we identified a soluble carbohydrate-binding protein, Galectin-1, as a candidate. We show herein that Galectin-1 is expressed in a subset of slowly dividing subventricular zone astrocytes, which includes the NSCs. Based on results from intraventricular infusion experiments and phenotypic analyses of knockout mice, we demonstrate that Galectin-1 is an endogenous factor that promotes the proliferation of NSCs in the adult brain.