Partial dissociation of molecular and behavioral measures of song habituation in adult zebra finches
Partial dissociation of molecular and behavioral measures of song habituation in adult zebra finches
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DOI：
10.1111/j.1601-183x.2008.00423.x
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发表时间：
2008-10-01
影响因子：
2.5
通讯作者：
Clayton, D. F.
中科院分区：
文献类型：
--
作者：
Dong, S.;Clayton, D. F.
最初回放记录的鸟鸣会触发斑雀的一系列反应，包括公开的倾听行为和依赖ERK通路的前脑听觉小叶Zenk基因转录的激活。重复一首歌的刺激会导致这些反应的持续习惯性，通过一天后的后续陈述来衡量。在这项研究中，我们研究了行为和分子(ERK/ZENK)习惯化之间的因果关系。在受试者内部的比较中，我们发现这两种反应(行为倾听的持续时间和Zenk表达的幅度)与先前训练的水平有很强的相关性，但同一组中的鸟类的这些反应之间的相关性很小。然后，我们验证了训练中ERK/Zenk的激活对于习惯化的发展是必要的这一假设，1天后测量。在训练前立即插管定向输注ERK激活的药理抑制剂(U0126)，阻止了Zenk基因反应的习惯性发展。然而，对行为习惯化影响的测量是混乱的，因为注射了非活性药物类似物(U0124)的鸟类在1天后表现出反应减弱，即使是对新歌。我们的结论是，对歌曲刺激的行为反应受到歌曲熟悉度以外的其他因素的强烈影响，而前脑的Zenk反应可能是听到特定歌曲的实际体验的更准确的指示器。
Initial playback of recorded birdsong triggers a number of responses in zebra finches, including overt listening behavior and ERK pathway-dependent activation of zenk gene transcription in the auditory lobule of the forebrain. Repetition of one song stimulus leads to persistent habituation of these responses, as measured by subsequent presentations 1 day later. In this study, we examined the causal relationships between behavioral and molecular (ERK/zenk) habituation. In a within-subject comparison, we found a strong correlation with the level of prior training for both responses (duration of behavioral listening and magnitude of zenk expression), but little correlation between these responses for birds within the same treatment group. We then tested the hypothesis that ERK/zenk activation during training is necessary for the development of habituation measured 1 day later. Cannula-directed infusion of a pharmacological inhibitor of ERK activation (U0126) immediately before training blocked the development of habituation of the zenk gene response. However, measurement of the effect on behavioral habituation was confounded because birds that were infused with a non-active drug analogue (U0124) showed a decreased response 1 day later, even to novel songs. We conclude that the behavioral response to song stimulation is strongly influenced by factors other than song familiarity, whereas the zenk response in the forebrain may be a more accurate indicator of actual experience hearing a particular song.