Distribution of Amidinohydrolases among Pseudomonas and Comparative Studies of Some Purified Enzymes by One-dimensional Peptide Mapping
Distribution of Amidinohydrolases among Pseudomonas and Comparative Studies of Some Purified Enzymes by One-dimensional Peptide Mapping
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假单胞菌中脒基水解酶的分布及部分纯化酶的一维肽图比较研究
DOI：
10.1271/bbb1961.47.2825
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
K. Yonaha
中科院分区：
文献类型：
--
作者：
T. Yorifuji;Toru Kobayashi;A. Tabuchi;Y. Shiritani;K. Yonaha
研究了胍基乙酸脒基水解酶（GAH）、3-胍基丙酸脒基水解酶（GPH）和4-胍基丁酸脒基水解酶（GBH）在10株荧光假单胞菌中的分布。GBH由大多数菌株产生，但GPH仅由铜绿假单胞菌菌株产生，GAH由三种恶臭假单胞菌菌株之一和假单胞菌ATCC 14676产生。在大多数产生GBH的细菌中，包括假单胞菌ATCC 14676，GBH由L-精氨酸以及由4-胍基丁酸诱导。相反，在两株铜绿假单胞菌和一株荧光假单胞菌中，该酶仅由4-胍基丁酸酯诱导。将铜绿假单胞菌PAO 1的GBH纯化至表观均一性，并通过一维肽图谱将酶蛋白与来自假单胞菌ATCC 14676的GBH蛋白进行比较。对枯草杆菌蛋白酶BPN '、嗜热菌蛋白酶、α-糜蛋白酶和金黄色葡萄球菌产生的两种蛋白质的裂解模式进行了比较。
The distribution of guanidinoacetate amidinohydrolase (GAH), 3-guanidinopropionate amid-inohydrolase (GPH) and 4-guanidinobutyrate amidinohydrolase (GBH) among ten representative strains of fluorescent Pseudomonas was examined. GBH was produced by most of the strains, but GPH was produced by Pseudomonas aeruginosa strains only, and GAH was produced by one of the three P. putida strains and by Pseudomonas sp. ATCC 14676. In most of the GBH-producing bacteria, including Pseudomonas sp. ATCC 14676, GBH was induced by l-arginine as well as by 4-guanidinobutyrate. In contrast, the enzyme was induced exclusively by 4-guanidinobutyrate in two strains of P. aeruginosa and a strain of P. fluorescens. GBH of P. aeruginosa PAO1 was purified to apparent homogeneity and the enzyme protein was compared with the GBH protein from Pseudomonas sp. ATCC 14676 by one-dimensional peptide mapping. The fragmentation patterns from the two proteins, generated by subtilisin BPN', thermolysin, α-chymotrypsin, and Staphylococcus aur...