Phospholipid hydroperoxide glutathione peroxidase: expression pattern during testicular development in mouse and evolutionary conservation in spermatozoa
Phospholipid hydroperoxide glutathione peroxidase: expression pattern during testicular development in mouse and evolutionary conservation in spermatozoa
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磷脂氢过氧化物谷胱甘肽过氧化物酶：小鼠睾丸发育过程中的表达模式和精子的进化保守
DOI：
10.1002/mrd.20039
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发表时间：
2004
影响因子：
2.5
通讯作者：
W. Engel
中科院分区：
文献类型：
--
作者：
K. Nayernia;M. Diaconu;G. Aumüller;G. Wennemuth;Iris Schwandt;K. Kleene;H. Kuehn;W. Engel
磷脂氢谷胱甘肽过氧化物酶（PHGPx）是谷胱甘肽过氧化物酶家族中的一种硒蛋白，参与抗氧化防御和精子发生。PHGPx几乎占了哺乳动物睾丸的全部硒含量。为了验证PHGPx的表达模式，用单克隆抗PHGPx抗体对生殖细胞发育不同阶段停滞的小鼠突变体的睾丸和不同年龄小鼠的睾丸进行免疫染色。PHGPx在各发育阶段睾丸间质细胞中均有表达。在生精小管中，PHGPx染色首先在21日龄小鼠的睾丸中观察到，这与第一个精子细胞的出现相关。当使用具有确定的生殖细胞发育停滞的突变小鼠的睾丸时，证实了该结果。在奥尔特/奥尔特和qk/qk小鼠的生精小管中观察到免疫染色，其显示精子细胞分化停滞。在从突变小鼠睾丸和发育中睾丸提取的蛋白质的Western印迹分析中，观察到19-和22-kDa的两个信号，证实睾丸细胞中存在两种PHGPx形式。在小鼠精子中，证明了PHGPx和精子线粒体相关富含半胱氨酸蛋白（SMCP）的亚细胞定位，表明PHGPx定位于精子中段的线粒体中。为了验证PHGPx在其他物种中的中段定位，使用抗PHGPx抗体对黑腹果蝇、青蛙、鱼、公鸡、小鼠、大鼠、猪、牛和人的精子进行免疫染色。PHGPx的定位被发现在中段的精子在所有物种的检查。电镜下可见中段线粒体内有PHGPx信号。这些结果表明PHGPx在精子功能和男性生育力中具有保守的关键作用。摩尔Reprod. Dev. 67：458-464，2004.© 2004 Wiley利斯公司
Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is a selenoprotein belonging to the family of glutathione peroxidases and has been implicated in antioxidative defense and spermatogenesis. PHGPx accounts for almost the entire selenium content of mammalian testis. In an attempt to verify the expression pattern of PHGPx, testes of mouse mutants with arrest at different stages of germ cell development and testes of mice at different ages were subjected to immunostaining with a monoclonal anti‐PHGPx antibody. PHGPx was detected in Leydig cells of testes in all developmental stages. In the seminiferous tubuli, the PHGPx staining was first observed in testes of 21‐day‐old mice which is correlated with the appearance of the first spermatids. This result was confirmed when the testes of mutant mice with defined arrest of germ cell development were used. An immunostaining was observed in the seminiferous tubuli of olt/olt and qk/qk mice which show an arrest at spermatid differentiation. In Western blot analysis of proteins extracted from testes of mutant mice and from developing testes, two signals at 19‐ and 22‐kDa were observed which confirm the existence of two PHGPx forms in testicular cells. In mouse spermatozoa, a subcellular localization of PHGPx and sperm mitochondria‐associated cysteine‐rich protein (SMCP) was demonstrated, indicating the localization of PHGPx in mitochondria of spermatozoa midpiece. For verifying the midpiece localization of PHGPx in other species, spermatozoa of Drosophila melanogaster, frog, fish, cock, mouse, rat, pig, bull, and human were used in immunostaining using anti‐PHGPx antibody. A localization of PHGPx was found in the midpiece of spermatozoa in all species examined. In electronmicroscopical analysis, PHGPx signals were found in the mitochondria of midpiece. These results indicate a conserved crucial role of PHGPx during sperm function and male fertility. Mol. Reprod. Dev. 67: 458–464, 2004. © 2004 Wiley‐Liss, Inc.
影响因子：
3.5
作者：
R. Esworthy;K. Doan;J. Doroshow;F. Chu
通讯作者：
R. Esworthy;K. Doan;J. Doroshow;F. Chu