Immunohistochemical analysis of apoptosis-related proteins in human embryonic and fetal pancreatic tissues
Immunohistochemical analysis of apoptosis-related proteins in human embryonic and fetal pancreatic tissues
复制标题
人胚胎和胎儿胰腺组织中凋亡相关蛋白的免疫组织化学分析
DOI：
--
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
M. Imamura
中科院分区：
文献类型：
--
作者：
Hiroyuki Kobayashi;R. Doi;R. Hosotani;Y. Miyamoto;T. Koshiba;K. Fujimoto;J. Ida;S. Tsuji;S. Nakajima;Michiya Kawaguchi;K. Shiota;M. Imamura
摘要背景。癌细胞和胎儿组织的生长都是快速的;然而，癌细胞以无序的方式去分化和增殖，而胎儿组织以有序的方式分化和增殖。因此，在胰腺癌的不受控制的细胞生长和胎儿胰腺的发育过程中可能存在共同和不同的因素。这些机制的共同部分应该是调节细胞周期，通过生长刺激和避免凋亡等机制导致快速增殖。因此，在本研究中，我们研究了胎儿胰腺组织中胰岛相关蛋白的表达。16人胚胎和胎儿胰腺组织之间获得的6和32周的妊娠。免疫组化检测Bcl-2、Bcl-XL、Mcl-1和Bax蛋白表达。检测胰岛素、胰高血糖素、增殖细胞核抗原（PCNA）的表达及TUNEL染色。在胚胎和胎儿胰腺组织中，Bcl-2在任何类型的胰腺细胞（腺泡、导管或胰岛）中均未检测到。Bcl-XL在整个妊娠期间在所有类型的胰腺细胞中表达。Mcl-1在各种类型的胰腺成分中均有表达，在胰岛边缘表达较强。Bax是一种促凋亡蛋白，在所有组分中均表达。PCNA在胚胎和胎儿胰腺中强表达，尤其是在妊娠早期;然而，TUNEL染色在所有样本中均为阴性。在所有类型的胰腺细胞中至少表达一种抗凋亡蛋白。目前的研究结果表明，积极的增殖和避免凋亡发生在胚胎和胎儿胰腺组织，这可能是由特定的组合控制的胰腺炎相关蛋白。其中Bcl-XL和Mcl-1可能在胚胎和胎儿胰腺的增殖和分化中起重要作用。
SummaryBackground. The growth of both cancer cells and fetal tissue is rapid; however, cancer cells de-differentiate and proliferate in a disorderly manner, whereas fetal tissues differentiate and proliferate in an orderly manner. Thus, there may be both common and different factors that are involved in the process of the uncontrolled cell growth of pancreatic cancers and the development of the fetal pancreas. The common part of the mechanisms should be in the regulation of the cell cycle, resulting in rapid proliferation via such mechanisms as growth stimulation and avoidance of apoptosis. Therefore, in the current study we investigated the expression of apoptosis-related proteins in fetal pancreatic tissues.Methods. Sixteen human embryonic and fetal pancreatic tissues obtained between 6 and 32 wk of gestation were used. We immunohistochemically examined the protein expression of Bcl-2, Bcl-XL, Mcl-1, and Bax. Further, the expression of insulin, glucagon, and proliferting cell nuclear antigen (PCNA), and TdT-mediated dUTP-biotin nick-end labeling (TUNEL) staining were examined.Results. In embryonic and fetal pancreatic tissues, Bcl-2 was not detected in any type of pancreatic cell (acinar, ductal, or islet). Bcl-XL was expressed in all types of pancreatic cells throughout the gestation. Mcl-1 was expressed in all types of pancreatic components, and strongly expressed in the margin of the islets. Bax, a pro-apoptotic protein, was expressed in all components. PCNA was strongly expressed in the embryonic and fetal pancreas, especially in early stages of gestation; however, TUNEL staining was negative in all samples. At least one antiapoptotic protein was expressed in all types of pancreatic cells.Conclusion. The results of the current study indicate that active proliferation and avoidance of apoptosis take place in embryonic and fetal pancreatic tissues, which may be controlled by particular combinations of apoptosis-related proteins. Among these proteins, Bcl-XL and Mcl-1 may play an important role in the proliferation and differentiation of the embryonic and fetal pancreas.
登录
查看更多内容
影响因子：
11.2
作者：
J. Reynolds;Tao Yang;Liping Qian;J. Jenkinson;Ping Zhou;Alan Eastman;Roger Craig
通讯作者：
J. Reynolds;Tao Yang;Liping Qian;J. Jenkinson;Ping Zhou;Alan Eastman;Roger Craig
影响因子：
11.2
作者：
M. Krajewska;S. Moss;S. Krajewski;K. Song;P. Holt;John Calvin Reed
通讯作者：
M. Krajewska;S. Moss;S. Krajewski;K. Song;P. Holt;John Calvin Reed
影响因子：
20.3
作者：
Zhou, P;Qian, LP;Craig, RW
通讯作者：
Craig, RW
DOI：
10.1073/pnas.92.10.4304
发表时间：
1995-05-09
影响因子：
11.1
作者：
GONZALEZGARCIA, M;GARCIA, I;NUNEZ, G
通讯作者：
NUNEZ, G
胃腺癌中 Bcl-2 家族蛋白的免疫组织化学分析。
DOI：
--
发表时间：
1996
期刊：
The American journal of pathology.
影响因子：
--
作者：
Krajewska,M;Fenoglio-Preiser,CM;Krajewski,S;Song,K;Macdonald,JS;Stemmerman,G;Reed,JC
通讯作者：
Reed,JC