Are inducible components involved in the repair of irradiated neuronal and brain tumour DNA?
Are inducible components involved in the repair of irradiated neuronal and brain tumour DNA?
复制标题
诱导成分是否参与受辐射神经元和脑肿瘤 DNA 的修复？
DOI：
10.1080/09553008114551211
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Ritter,P
中科院分区：
文献类型：
--
作者：
Wheeler,KT;Wierowski,JV;Ritter,P
2. Materials and methods The entire heads of anaesthetized, 240-280 g (3-4 month old), male Fisher 344 rats bearing 100-200 mg intracerebral 9L brain tumours were irradiated with single doses of 12-5 or 25.0 Gy of Cs-137 y-rays at a dose rate of 9-21 Gy/min. The remainder of the body was shielded with lead. At various times after irradiation, the rats were killed by decapitation and the tumour and cerebellum were removed. The tumour and cerebellum were dissociated into a single cell suspension at 4 C by mechanical means and the cell suspensions layered onto a lytic zone over a 10-30% alkaline sucrose gradient in two Beckman Ti-15 zonal rotors. After sedimentation the DNA was collected in 20 ml fractions, precipitated with perchloric acid, reacted with diaminobenzoic acid dihydrochloride and the amount of DNA in each fraction determined by spectrofluorometrically measuring the fluorescence emitted at 500 nm after excitation at 409 rim. Complete descriptions of the tumour implantation procedure, tumour growth characteristics, dissociation procedure, gradients, sedimentation conditions, typical sedimentation profiles and the molecular properties of the sedimenting DNA have been published (Wang and Wheeler 1978, Leith et al. 1975, Wheeler and Wallen 1980, Wheeler et al. 1975). The percentage of the DNA with sedimentation coefficients> 250S was calculated from the DNA sedimentation profiles of both irradiated and unirradiated
