Comparison of gravimetric, creamatocrit and esterified fatty acid methods for determination of total fat content in human milk
Comparison of gravimetric, creamatocrit and esterified fatty acid methods for determination of total fat content in human milk
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DOI：
10.1016/j.foodchem.2016.08.114
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发表时间：
2017-02-15
期刊：
影响因子：
8.8
通讯作者：
Geddes, Donna T.
中科院分区：
文献类型：
--
作者：
Du, Jian;Gay, Melvin C. L.;Geddes, Donna T.
重量法被认为是测量人乳脂肪含量的金标准。然而，它是劳动密集型的，需要大量的人乳。其他方法，如乳脂比容和酯化脂肪酸测定（EFA），也已广泛用于脂肪分析。然而，这些方法尚未与重量法同时进行比较。用不同脂肪含量的人乳对这三种方法进行了比较。这些方法之间的相关性很高（r（2）= 0.99）。使用这三种方法，观察到总体脂肪测量值和各组（低、中、高脂肪牛奶）内的统计学差异（P < 0.001）。总体而言，与重量法相比，观察到乳脂压积法与EFA法的相关性更强，平均值（4.73 g/L）和百分比差异（5.16%）更低。此外，易于操作和实时分析使得乳脂比容法更可取。(C)2016由Elsevier Ltd.出版
The gravimetric method is considered the gold standard for measuring the fat content of human milk. However, it is labor intensive and requires large volumes of human milk. Other methods, such as creamatocrit and esterified fatty acid assay (EFA), have also been used widely in fat analysis. However, these methods have not been compared concurrently with the gravimetric method. Comparison of the three methods was conducted with human milk of varying fat content. Correlations between these methods were high (r(2) = 0.99). Statistical differences (P < 0.001) were observed in the overall fat measurements and within each group (low, medium and high fat milk) using the three methods. Overall, stronger correlation with lower mean (4.73 g/L) and percentage differences (5.16%) was observed with the creamatocrit than the EFA method when compared to the gravimetric method. Furthermore, the ease of operation and real-time analysis make the creamatocrit method preferable. (C) 2016 Published by Elsevier Ltd.