Axonal Collateral Branching of Neurones in the Inferior Olive Projecting to the Cerebellar Paramedian Lobule in the Rabbit
Axonal Collateral Branching of Neurones in the Inferior Olive Projecting to the Cerebellar Paramedian Lobule in the Rabbit
复制标题
兔下橄榄神经元轴突侧支分支投射到小脑旁正中小叶
DOI：
10.1159/000064384
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发表时间：
2002
影响因子：
2.7
通讯作者：
B. Mierzejewska
中科院分区：
文献类型：
--
作者：
D. Bukowska;L. Zguczyński;B. Mierzejewska
采用双荧光逆行技术，观察了兔小脑旁小叶（PML）吻侧和尾侧皮层下橄榄（IO）神经元的分布，分别为面部-前肢和后肢接收区。此外，该技术还用于研究到达这两个体位非同源PML区域的IO投射是否可能是同一轴突的侧枝。没有其他报告涉及这个问题。将细胞质示踪剂快蓝（FB）和核染料二氨基黄（DY）分别注射到吻侧和尾侧PML后，在对侧IO的特定区域发现了许多单个FB或DY标记的细胞。这些区域显示出相当大的重叠，除了背侧副橄榄，在标记的细胞群中观察到明确的空间分离。此外，双FB + y标记的神经元（n = 310）分布于内侧副橄榄、主橄榄的背侧和腹侧板、背内侧细胞柱和β核。提示IO神经元可能分支供应两个功能不同的PML区域，从而参与前肢-后肢协调机制。
The double fluorescent retrograde technique was employed to examine the distribution of the inferior olive (IO) neurones projecting to the cortex of the rostral and caudal parts of the paramedian lobule (PML) in the rabbit cerebellum, known to be the face-forelimb and hindlimb receiving areas, respectively. Moreover, this technique was also used to investigate the possibility that IO projections reaching these two somatotopically non-homologous PML regions are collaterals of the same axones. No other reports have addressed this question. After non-overlapping unilateral injections of the cytoplasmic tracer fast blue (FB) and the nuclear dye diamidino yellow (DY) into the rostral and caudal PML, respectively, numerous single FB- or DY-labelled cells were found in the defined regions of the contralateral IO. These regions showed considerable overlap, apart from the dorsal accessory olive where a clear spatial separation of labelled cell groups was observed. Furthermore, double FB + DY-labelled neurones (n = 310) were seen in the medial accessory olive, the dorsal and ventral laminas of the principal olive, in the dorsomedial cell column and the β nucleus. It suggests that IO neurones may branch to supply the two functionally different PML regions and in this way participate in the mechanisms of forelimb-hindlimb coordination.