INO80 exchanges H2A.Z for H2A by translocating on DNA proximal to histone dimers.
INO80 exchanges H2A.Z for H2A by translocating on DNA proximal to histone dimers.
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DOI：
10.1038/ncomms15616
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发表时间：
2017-06-12
影响因子：
16.6
通讯作者：
Bartholomew B
中科院分区：
文献类型：
--
作者：
Brahma S;Udugama MI;Kim J;Hada A;Bhardwaj SK;Hailu SG;Lee TH;Bartholomew B
ATP依赖的染色质重塑通过移动或拆卸核小体以及改变核小体组成来调节核小体动力学。这些染色质重塑物通常通过在核小体的H3-H4界面处沿核小体DNA沿着易位而起作用。在这里，我们表明，与其他重塑，INO 80易位沿着DNA在H2 A-H2 B界面的核小体，并持续取代DNA从表面的H2 A-H2 B。INO 80在核小体进入位点附近产生的DNA易位和DNA扭转应变促进核小体的动员和H2 A、Z-H2 B二聚体选择性地交换出核小体并被H2 A-H2 B二聚体取代，而无需任何额外的组蛋白伴侣。我们发现INO 80易位和动员H2 A. Z的核小体比那些含有H2 A，部分解释INO 80的偏好，以取代H2 A. Z与H2 A。我们的数据表明，INO 80有一个二聚体交换的机制，这是不同于其他染色质重塑，包括其paraminosSWR 1。染色质重塑剂通常通过在H3-H4界面处沿着核小体DNA移位来移动或分解核小体。在这里，作者提供了染色质重塑剂INO 80在H2 A-H2 B界面处沿沿着DNA易位并从H2 A-H2 B表面置换DNA的证据。
ATP-dependent chromatin remodellers modulate nucleosome dynamics by mobilizing or disassembling nucleosomes, as well as altering nucleosome composition. These chromatin remodellers generally function by translocating along nucleosomal DNA at the H3–H4 interface of nucleosomes. Here we show that, unlike other remodellers, INO80 translocates along DNA at the H2A–H2B interface of nucleosomes and persistently displaces DNA from the surface of H2A–H2B. DNA translocation and DNA torsional strain created near the entry site of nucleosomes by INO80 promotes both the mobilization of nucleosomes and the selective exchange of H2A.Z–H2B dimers out of nucleosomes and replacement by H2A–H2B dimers without any additional histone chaperones. We find that INO80 translocates and mobilizes H2A.Z-containing nucleosomes more efficiently than those containing H2A, partially accounting for the preference of INO80 to replace H2A.Z with H2A. Our data suggest that INO80 has a mechanism for dimer exchange that is distinct from other chromatin remodellers including its paralogue SWR1. Chromatin remodellers usually mobilize or disassemble nucleosomes by translocating along the nucleosomal DNA at the H3-H4 interface. Here, the authors provide evidence chromatin remodeller INO80 translocates along DNA at the H2A-H2B interface and displaces DNA from the surface of H2A-H2B.