The role of periostin in eosinophilic otitis media
The role of periostin in eosinophilic otitis media
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DOI：
10.3109/00016489.2012.668708
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发表时间：
2012-08-01
影响因子：
1.4
通讯作者：
Shinkawa, Hideichi
中科院分区：
文献类型：
--
作者：
Nishizawa, Hisanori;Matsubara, Atsushi;Shinkawa, Hideichi
结论：我们研究了Periostin在嗜酸性中耳炎(EOM)患者和新建立的EOM动物模型的中耳标本中的定位。在EOM患者的中耳切片上观察到Periostin免疫阳性染色。在动物模型中也观察到了Periostin的免疫反应。这些结果提示Periostin在EOM中耳上皮下纤维化中起重要作用。目的：探讨Periostin在EOM患者中耳及动物模型中的作用。方法：对6例伴/不伴哮喘的EOM患者的中耳标本进行组织学和免疫组织化学分析。用卵清蛋白(OVA)腹腔内致敏和局部致敏建立EOM动物模型。在该模型中还进行了Periostin的组织学和免疫细胞化学分析。结果：所有伴有或不伴有哮喘的EOM患者中耳切片的基底膜和细胞外基质均有Periostin免疫反应阳性。在动物模型中，观察到嗜酸性粒细胞的浸润和中耳粘膜厚度。此外，在OVA增强侧中耳粘膜的细胞外基质中可见Periostin阳性免疫染色。
Conclusion: We investigated the localization of periostin in middle ear specimens from patients with eosinophilic otitis media (EOM) and from a newly constructed animal model for EOM. Periostin-positive immunostaining was observed in the middle ear sections obtained from the EOM patients. Immunoreactivity for periostin was also seen in the animal model. These results suggest that periostin plays an important role in subepithelial fibrosis in the middle ear in EOM. Objective: The purpose of the present study was to investigate the role of periostin in the middle ear of EOM patients and an animal model. Methods: Histological and immunohistochemical analyses for periostin were carried out in the middle ear specimens of six EOM patients with/without asthma. An animal model of EOM was constructed by intraperitoneal and topical sensitization with ovalbumin (OVA). Histological and immunocytochemical analyses for periostin were also performed in this model. Results: Immunoreactivities for periostin were observed in the basement membrane and extracellular matrix of the middle ear sections obtained from all EOM patients with/without asthma. In the animal model, eosinophil infiltration and middle ear mucosa thickness were observed. Moreover, periostin-positive immunostaining was shown in the extracellular matrix of the middle ear mucosa on the side topically boosted by OVA.