Barrel Jellyfish (Rhizostoma pulmo) as Source of Antioxidant Peptides
Barrel Jellyfish (Rhizostoma pulmo) as Source of Antioxidant Peptides
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DOI：
10.3390/md17020134
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发表时间：
2019-02-01
期刊：
影响因子：
5.4
通讯作者：
Leone, Antonella
中科院分区：
文献类型：
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作者：
De Domenico, Stefania;De Rinaldis, Gianluca;Leone, Antonella
在地中海沿岸沃茨，水母Rupstoma pulmo，Macri 1778（刺胞动物，Rupstomae）经历了反复爆发，大量的生物量种群对海洋和海上活动造成了滋扰或损害。本文综述了近年来国内外对红曲霉抗氧化活性的研究进展。本文基于可溶性和不溶性膜分级蛋白的提取和表征提供了肺蛋白质化合物，后者通过用胃蛋白酶和胶原酶连续酶促水解来消化。所有的水母蛋白质表现出显着的AA，低分子量（MW）的蛋白质与更大的抗氧化活性。特别地，胶原酶水解的胶原产生MW低于3 kDa的肽，范围为3-10 kDa或10-30 kDa，AA与MW成反比。在0.05-20 μ g/mL的蛋白质浓度范围内，除了高于30 kDa的可溶性蛋白质（可能含有水母毒液化合物）外，所有测试的蛋白质级分对培养的人角质形成细胞（HEKa）均未检测到细胞毒性作用。此外，水解的水母胶原蛋白肽显示出显着更高的AA，并提供了更大的保护作用，对HEKa氧化应激比脊椎动物的水解胶原蛋白肽。由于具有很高的繁殖潜力，水母作为天然生物活性化合物的来源可能代表着潜在的社会经济机会，具有深远的有益影响。最终，加工技术的改进将促进未开发的海洋生物质在营养品，化妆品和制药领域的使用，将海洋管理问题转化为更积极的观点。
The jellyfish Rhizostoma pulmo, Macri 1778 (Cnidaria, Rhizostomae) undergoes recurrent outbreaks in the Mediterranean coastal waters, with large biomass populations representing a nuisance or damage for marine and maritime activities. A preliminary overview of the antioxidant activity (AA) of R. pulmo proteinaceous compounds is provided here based on the extraction and characterization of both soluble and insoluble membrane-fractioned proteins, the latter digested by sequential enzymatic hydrolyses with pepsin and collagenases. All jellyfish proteins showed significant AA, with low molecular weight (MW) proteins correlated with greater antioxidant activity. In particular, collagenase-hydrolysed collagen resulted in peptides with MW lower than 3 kDa, ranging 3-10 kDa or 10-30 kDa, with AA inversely proportional to MW. No cytotoxic effect was detected on cultured human keratinocytes (HEKa) in a range of protein concentration 0.05-20 g/mL for all tested protein fractions except for soluble proteins higher than 30 kDa, likely containing the jellyfish venom compounds. Furthermore, hydrolyzed jellyfish collagen peptides showed a significantly higher AA and provided a greater protective effect against oxidative stress in HEKa than the hydrolyzed collagen peptides from vertebrates. Due to a high reproductive potential, jellyfish may represent a potential socioeconomic opportunity as a source of natural bioactive compounds, with far-reaching beneficial implications. Eventually, improvements in processing technology will promote the use of untapped marine biomasses in nutraceutical, cosmeceutical, and pharmaceutical fields, turning marine management problems into a more positive perspective.