Microbial Reductive Dechlorination of Aroclor 1260 in Anaerobic Slurries of Estuarine Sediments
Microbial Reductive Dechlorination of Aroclor 1260 in Anaerobic Slurries of Estuarine Sediments
复制标题
Aroclor 1260 在河口沉积物厌氧泥浆中的微生物还原脱氯
DOI：
10.1128/aem.64.3.1052-1058.1998
复制
发表时间：
1998
影响因子：
4.4
通讯作者：
H. May
中科院分区：
文献类型：
--
作者：
Qingzhong Wu;K. Sowers;H. May
摘要研究了多氯联苯1260在巴尔的摩港（巴尔的摩，马里兰州）河口沉积物厌氧泥浆中的还原脱氯作用。在添加和不添加350 μM 2，3，4，5-四氯联苯（2，3，4，5-CB）或2，3，5，6-四氯联苯（2，3，5，6-CB）的情况下，用800 ppm Aroclor 1260对沉积物浆液进行改良，并在30°C下在人工河口培养基中的产甲烷条件下一式三份孵育。6个月后，除灭菌对照外，所有孵育培养物中均发生Aroclor 1260的广泛脱氯和中度邻位脱氯。总体而言，每个联苯的总氯含量减少了高达34%。当提供2，3，4，5-CB、2，3，5，6-CB或不提供额外同系物时，每联苯基的Meta氯分别减少65%、55%和45%，邻位氯分别减少18%、12%和9%。这是第一份经确认的商用多氯联苯混合物微生物邻位脱氯的报告。此外，与单独提供Aroclor 1260的培养物相比，Aroclor 1260加2，3，4，5-CB或2，3，5，6-CB的脱氯滞后时间更短（31至60天，而不是90天），而且脱氯时间更长，这表明添加单一同系物可刺激Aroclor 1260的脱氯。
ABSTRACT Reductive dechlorination of Aroclor 1260 was investigated in anaerobic slurries of estuarine sediments from Baltimore Harbor (Baltimore, Md.). The sediment slurries were amended with 800 ppm Aroclor 1260 with and without the addition of 350 μM 2,3,4,5-tetrachlorobiphenyl (2,3,4,5-CB) or 2,3,5,6-tetrachlorobiphenyl (2,3,5,6-CB) and incubated in triplicate at 30°C under methanogenic conditions in an artificial estuarine medium. After 6 months, extensivemeta dechlorination and moderate orthodechlorination of Aroclor 1260 occurred in all incubated cultures except for sterilized controls. Overall, total chlorines per biphenyl decreased by up to 34%. meta chlorines per biphenyl decreased by 65, 55, and 45% and ortho chlorines declined by 18, 12, and 9%, respectively, when 2,3,4,5-CB, 2,3,5,6-CB, or no additional congener was supplied. This is the first confirmed report of microbial ortho dechlorination of a commercial polychlorinated biphenyl mixture. In addition, compared with incubated cultures supplied with Aroclor 1260 alone, the dechlorination of Aroclor 1260 plus 2,3,4,5-CB or 2,3,5,6-CB occurred with shorter lag times (31 to 60 days versus 90 days) and was more extensive, indicating that the addition of a single congener stimulated the dechlorination of Aroclor 1260.