Targeting and assembly of the oxoglutarate carrier: general principles for biogenesis of carrier proteins of the mitochondrial inner membrane.
Targeting and assembly of the oxoglutarate carrier: general principles for biogenesis of carrier proteins of the mitochondrial inner membrane.
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氧化戊二酸载体的靶向和组装：线粒体内膜载体蛋白生物发生的一般原理。
DOI：
10.1042/bj3330151
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Ferdinando Palmieri
中科院分区：
文献类型：
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作者：
Annamaria Palmisano;Vincenzo Zara;A. Hönlinger;A. Vozza;Peter J. T. Dekker;N. Pfanner;Ferdinando Palmieri
我们已经研究了2-酮戊二酸载体（OGC），一个完整的线粒体内膜蛋白的靶向和组装。OGC的前体，合成没有可裂解的前序列，被转运到线粒体中的ATP和膜电位依赖性的方式。哺乳动物OGC的输入有效地发生到哺乳动物和酵母线粒体中。OGC的靶向揭示了对线粒体表面受体Tom 70（线粒体外膜转位酶的70 kDa亚基）的明显依赖，而可裂解的前蛋白依赖于Tom 20（20 kDa亚基），支持受体特异性差异的模型和线粒体不同靶向途径的存在。微量的OGC在体外导入到二聚体形式的组装可以通过毛地黄皂苷裂解的线粒体的蓝色天然电泳来监测。哺乳动物OGC和真菌ADP/ATP载体的组装在哺乳动物和酵母线粒体中都以高效率发生。这些研究结果表明，在线粒体内膜的载体二聚体的动态行为，并建议从哺乳动物到真菌的组装反应的高度保守。
We have studied the targeting and assembly of the 2-oxoglutarate carrier (OGC), an integral inner-membrane protein of mitochondria. The precursor of OGC, synthesized without a cleavable presequence, is transported into mitochondria in an ATP- and membrane potential-dependent manner. Import of the mammalian OGC occurs efficiently into both mammalian and yeast mitochondria. Targeting of OGC reveals a clear dependence on the mitochondrial surface receptor Tom70 (the 70 kDa subunit of the translocase of the outer mitochondrial membrane), whereas a cleavable preprotein depends on Tom20 (the 20 kDa subunit), supporting a model of specificity differences of the receptors and the existence of distinct targeting pathways to mitochondria. The assembly of minute amounts of OGC imported in vitro to the dimeric form can be monitored by blue native electrophoresis of digitonin-lysed mitochondria. The assembly of mammalian OGC and fungal ADP/ATP carrier occurs with high efficiency in both mammalian and yeast mitochondria. These findings indicate a dynamic behaviour of the carrier dimers in the mitochondrial inner membrane and suggest a high conservation of the assembly reactions from mammals to fungi.