DEEP GREEN PANICLE1 suppresses GOLDEN2-LIKE activity to reduce chlorophyll synthesis in rice glumes
DEEP GREEN PANICLE1 suppresses GOLDEN2-LIKE activity to reduce chlorophyll synthesis in rice glumes
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DEEP GREEN PANICLE1 抑制 GOLDEN2-LIKE 活性，减少水稻颖片中叶绿素的合成
DOI：
10.1093/plphys/kiaa038
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发表时间：
2021
期刊：
影响因子：
7.4
通讯作者：
Yu Hengxiu
中科院分区：
文献类型：
--
作者：
Zhang Chao;Zhang Jianxiang;Tang Yujie;Liu Kangwei;Liu Yan;Tang Jiaqi;Zhang Tao;Yu Hengxiu
了解光合作用的调节机制是提高其效率并最终提高作物产量的关键。在这项研究中，我们报告了绿色PANICLE 1（DGP 1）参与水稻（Oryza sativa L.）光合作用的调节。我们鉴定了dgp 1突变体，它增加了颖片中叶绿素含量。通过图位克隆的方法分离突变基因。使用基因编辑方法产生的敲除植物模仿dgp 1的表型。DGP 1的过量表达导致叶片和颖片失绿。DGP 1是一种植物特异性蛋白，具有保守的TIGR 01589结构域。在绿色组织中检测到DGP 1的表达，其表达受光诱导。此外，参与叶绿素合成关键步骤的基因在dgp 1的颖片中被上调。重要的是，我们发现DGP 1与水稻蛋白GOLDEN 2-LIKE 1（OsGLK 1）和GOLDEN 2-LIKE 2（OsGLK 2）相互作用，这两种转录因子参与光合作用的调节。反式激活实验表明，DGP 1抑制OsGLK 1对靶基因的激活活性。我们的研究结果表明，DGP 1是OsGLK活性的阻遏物，从而在水稻光合作用。对该基因及其同源基因在其他作物中的操纵可能为高光效育种提供新的途径。
Understanding the regulation mechanisms of photosynthesis is key to improving its efficiency and, ultimately, crop yield. In this study, we report that DEEP GREEN PANICLE1 (DGP1) is involved in photosynthesis regulation in rice (Oryza sativaL.). We identified thedgp1mutant, which has increased chlorophyll content in glumes. The mutated gene was isolated by map-based cloning. Knockout plants, generated using a gene editing approach, mimic the phenotype ofdgp1. Overexpression ofDGP1leads to chlorotic leaves and glumes. DGP1 is a plant-specific protein with a conserved TIGR01589 domain. The expression ofDGP1was detected in green tissues and is induced by light. Moreover, genes involved in key steps of chlorophyll synthesis are upregulated in the glumes ofdgp1. Importantly, we found that DGP1 interacts with the rice proteins GOLDEN2-LIKE1 (OsGLK1) and GOLDEN2-LIKE2 (OsGLK2), the two transcription factors involved in the regulation of photosynthesis. Transactivation assays showed that DGP1 represses the activation activity of OsGLK1 on its target genes. Our results demonstrate that DGP1 is a repressor of OsGLK activity and thus photosynthesis in rice. Manipulation of this gene and its homologs in other crops may provide new approaches for high photosynthetic efficiency breeding.