Differential expression of estrogen receptors alpha and beta mRNA during differentiation of human osteoblast SV-HFO cells
Differential expression of estrogen receptors alpha and beta mRNA during differentiation of human osteoblast SV-HFO cells
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DOI：
10.1210/en.138.11.5067
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发表时间：
1997-11-01
期刊：
影响因子：
4.8
通讯作者：
VanLeeuwen, JPTM
中科院分区：
文献类型：
--
作者：
Arts, J;Kuiper, GGJM;VanLeeuwen, JPTM
雌激素已被证明是维持足够高的骨矿密度所必需的，而且已证实ERα在骨细胞中表达。最近，一种新的雌激素受体--雌激素受体β(ERβ)被鉴定出来。在这里，我们证明ERβ在人成骨细胞中也有表达，并且ERα和ERβ在人成骨细胞分化过程中有不同的表达。成骨细胞培养21d(矿化期)ERβmRNA表达逐渐增加，较第6天(增殖期)平均增加9.9+/-5.3倍(平均+/-sd，n=3)。相反，ERαmRNA的表达水平在第10天仅略有增加(2.3+/-1.7倍)，然后保持不变。观察到的ERα和ERβ的不同调控提示ERβ和ERα在骨代谢中具有额外的功能作用。
Estrogens have been shown to be essential for maintaining a sufficiently high bone mineral density and ER alpha expression has been demonstrated in bone cells. Recently, a novel estrogen receptor, estrogen receptor beta (ER beta) has been identified. Here we demonstrate that also ER beta is expressed in human osteoblasts, and that ER alpha and ER beta are differentially expressed during human osteoblast differentiation. ER beta mRNA expression increased gradually during osteoblast culture, resulting in an average increase of 9.9 +/- 5.3 fold (mean +/- S.D., n = 3) at day 21 (mineralization phase) as compared to day 6 (proliferation phase). In contrast, ER alpha mRNA expression levels increased only slightly until day 10 (2.3 +/- 1.7 fold) and then remained constant. The observed differential regulation of ER alpha and beta is suggestive for an additional functional role of ER beta to ER alpha in bone metabolism.