CELL LINEAGE OF ZEBRAFISH BLASTOMERES .1. CLEAVAGE PATTERN AND CYTOPLASMIC BRIDGES BETWEEN CELLS
CELL LINEAGE OF ZEBRAFISH BLASTOMERES .1. CLEAVAGE PATTERN AND CYTOPLASMIC BRIDGES BETWEEN CELLS
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DOI：
10.1016/0012-1606(85)90010-7
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发表时间：
1985-01-01
影响因子：
2.7
通讯作者：
LAW, RD
中科院分区：
文献类型：
--
作者：
KIMMEL, CB;LAW, RD
本文描述了斑马鱼胚胎中卵裂球之间的分裂和细胞质连接的模式。细胞分裂模式通常非常规则；在许多胚胎中，可以从S卵裂球在簇中的位置到细胞期来确定它的谱系。然而，在5h的切割中，观察到了显著的模式变化，并描述了第5次切割的替代模式。早期的卵裂是部分的，不完全地将卵裂球与巨大的卵黄细胞分开。将示踪剂荧光素-葡聚糖(FD)注射到卵裂球，以了解细胞质桥接的程度。直到第10次卵裂，位于胚胚层边缘的卵裂球仍保持着与卵黄细胞的细胞质桥梁。从第5次卵裂开始，注射到非边缘蓝孔的FD仍然局限于注射的细胞，或者如果注射是在细胞周期的早期，示踪剂通过从前一次卵裂延续的桥梁扩散到S兄弟细胞。注射的荧光黄可能通过缝隙连接在卵裂球中广泛传播，模式与血统无关。
Patterns of cleavage and cytoplasmic connections between blastomeres in the embryo of the zebrafish, B. rerio were described. The cell division pattern was often very regular; in many embryos a blastomere''s lineage may be ascertained from its position in the cluster through the 64-cell stage. At the 5h cleavage, however, significant variability in pattern was observed and alternative patterns of the 5th cleavage are described. The early cleavages were partial, incompletely separating blastomeres from the giant yolk cell. The tracer fluorescein-dextran (FD) was injected into blastomeres to learn the extent of the cytoplasmic bridging. Until the 10th cleavage, blastomeres located along the blastoderm margin maintained cytoplasmic bridges to the yolk cell. Beginning with the 5th cleavage, FD injected into a nonmarginal blustomere remains confined to the injected cell, or if the injection was early in the cell cycle, the tracer spreads to the cell''s sibling, through a bridge persisting from the previous cleavage. Injected Lucifer yellow spreads, presumably via gap junctions, widely among blastomeres in a pattern unrelated to lineage.