Genetic and functional characterization of the LTR of HIV-1 subtypes A and C circulating in India
Genetic and functional characterization of the LTR of HIV-1 subtypes A and C circulating in India
复制标题
DOI：
10.1089/aid.2007.0152
复制
发表时间：
2007-11-01
影响因子：
1.5
通讯作者：
Gupta, Phalguni
中科院分区：
文献类型：
--
作者：
Rodriguez, Milka A.;Shen, Chengli;Gupta, Phalguni
对印度不同地区流行的 HIV-1 序列的遗传分析表明，印度流行的 HIV-1 亚型的主要比例是 C 型，一小部分是 A、B、E 和 CRF 亚型。我们对从印度两个主要城市获得的 7 种 C 亚型和 5 种 A 亚型分离株的 HIV-1 LTR 启动子区域进行了测序。完整启动子和 TAR 区域的序列分析揭示了几个主要结合位点的保守亚型特异性变异性。所有 C 亚型分离株中均存在 3 个 NF-κ B 位点，并且两个分离株在 MFNLP 中包含插入。这些分离株之一的转录活性可能由于这种插入而受到阻碍。尽管 LTR 之间存在明显的变异性，但我们没有观察到 C 亚型和 A 亚型之间的转录活性有任何显着差异。据我们所知，这是第一项表征印度 A 亚型 LTR 的遗传结构和功能属性的研究。
Genetic analysis of HIV-1 sequences circulating in different parts of India have shown that the predominant proportion of HIV-1 subtypes circulating in India is type C and a small fraction are subtypes A, B, E, and CRFs. We sequenced the HIV-1 LTR promoter region of seven subtype C and five subtype A isolates obtained from two major cities in India. Sequence analysis of the complete promoter and TAR regions revealed conserved subtype-specific variability in several major binding sites. Three NF-kappa B sites were present in all subtype C isolates and two isolates contained an insertion in the MFNLP. The transcriptional activity of one of these isolates may have been hindered due to this insertion. Despite the apparent variability between the LTRs we did not observe any significant difference in the transcriptional activity between subtype C and subtype A. To our knowledge, this is the first study characterizing the genetic structure and functional attributes of subtype A LTRs from India.