Lymantria dispar iflavirus 1 (LdIV1), a new model to study iflaviral persistence in lepidopterans
Lymantria dispar iflavirus 1 (LdIV1), a new model to study iflaviral persistence in lepidopterans
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DOI：
10.1099/vir.0.067710-0
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发表时间：
2014-10-01
影响因子：
3.8
通讯作者：
Bonning, Bryony C.
中科院分区：
文献类型：
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作者：
Carrillo-Tripp, Jimena;Krueger, Elizabeth N.;Bonning, Bryony C.
来自舞毒蛾（Lymantria dispar L.）的IPLB-LD-652Y细胞系通常用于研究病毒与昆虫宿主之间的相互作用。本文报道了一种被发现持续感染IPLB-LD-652Y的小RNA病毒Lymantria dispar iflavirus 1 （LdIV1）的全基因序列和生物学特性。LdIV1属于黄病毒属。LdIV1形成的二十面体粒子约为。直径为30 nm，含有一个10044 nt的聚腺苷化正义RNA基因组，编码一个预测的2980 aa的多蛋白。LdIV1是由RNA沉默的病毒抑制因子诱导的，提示急性感染受到RNA干扰（RNA）的限制。我们在所有测试的舞毒蛾幼虫和成虫组织中检测到LdIV1，但在其他舞毒蛾衍生的细胞系中没有检测到该病毒。我们在其中两个细胞系（IPLB-LD-652和IPLB-LdFB）中证实了LdIV1的感染性。我们的研究结果为探索鳞翅目动物的持续感染提供了一个新的系统，并为研究黄病毒提供了一个新的模型。黄病毒是一种快速扩展的病毒群，其中许多病毒会秘密感染它们的宿主。
The cell line IPLB-LD-652Y, derived from the gypsy moth (Lymantria dispar L.), is routinely used to study interactions between viruses and insect hosts. Here we report the full genonne sequence and biological characteristics of a small RNA virus, designated Lymantria dispar iflavirus 1 (LdIV1), that was discovered to persistently infect IPLB-LD-652Y. LdIV1 belongs to the genus lflavirus. LdIV1 formed icosahedral particles of approx. 30 nm in diameter and contained a 10 044 nt polyadenylated, positive-sense RNA genome encoding a predicted polyprotein of 2980 aa. LdIV1 Was induced by a viral suppressor of RNA silencing, suggesting that acute infection is restricted by RNA interference (RNA. We detected LdIV1 in all tested tissues of gypsy-moth larvae and adults, but the virus was absent from other L. dispar-derived cell lines. We confirmed LdIV1 infectivity in two of these cell lines (IPLB-LD-652 and IPLB-LdFB). Our results provide a novel system to explore persistent infections in lepidopterans and a new model for the study of iflaviruses, a rapidly expanding group of viruses, many of which covertly infect their hosts.