Rice and Arabidopsis homologs of yeast CHROMOSOME TRANSMISSION FIDELITY PROTEIN 4 commonly interact with Polycomb complexes but exert divergent regulatory functions
Rice and Arabidopsis homologs of yeast CHROMOSOME TRANSMISSION FIDELITY PROTEIN 4 commonly interact with Polycomb complexes but exert divergent regulatory functions
复制标题
酵母染色体传输保真蛋白 4 的水稻和拟南芥同源物通常与 Polycomb 复合物相互作用，但发挥不同的调节功能
DOI：
10.1093/plcell/koab047
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发表时间：
2021
期刊：
影响因子：
11.6
通讯作者：
Gu Xiaofeng
中科院分区：
文献类型：
--
作者：
Zhang Pingxian;Zhu Chunmei;Geng Yuke;Wang Yifan;Yang Ying;Liu Qing;Guo Weijun;Chachar Sadaruddin;Riaz Adeel;Yan Shuangyong;Yang Liwen;Yi Keke;Wu Changyin;Gu Xiaofeng
在细胞分裂过程中，遗传和表观遗传信息都必须从母细胞传递到子细胞。关于染色质状态和表观遗传标记（如组蛋白3赖氨酸27三甲基化（H3K27me3））的信息传递机制已经在动物中被表征；这些过程在植物中还不太清楚。在此，基于矮水稻（Oryza sativa）突变体（矮相关wd40蛋白1，drw1）缺乏酵母ctf4（染色体传递保真蛋白4）的特征，我们发现植物中的ctf4同源物使用共同的细胞机制，但实现不同的功能结果。具体来说，drw1没有表现出开花相关的表型（如假定的同源拟南芥eol1突变体），但表现出细胞周期阻滞和DNA损伤反应。在机制上，我们证明DRW1通过调节细胞周期抑制剂skp相关蛋白1（KRP1）和krp5的表达来维持正常的细胞周期进程，并表明这些作用是由DRW1结合它们的启动子和增加H3K27me3水平介导的。因此，虽然拟南芥中的LHP1 1（EOL1）的ctf4 orthologsenhancer和水稻中的drw1在分裂细胞中都有独特的表达，通常与多个Polycomb复合物亚基相互作用，并促进H3K27me3沉积，但我们现在知道它们的调节功能在植物进化过程中存在很大的差异。此外，我们的工作通过实验阐明了CTF4/EOL1/DRW1的特定靶点，它们的蛋白-蛋白相互作用伙伴，以及它们在植物中的染色质/表观遗传效应。
Both genetic and epigenetic information must be transferred from mother to daughter cells during cell division. The mechanisms through which information about chromatin states and epigenetic marks like histone 3 lysine 27 trimethylation (H3K27me3) are transferred have been characterized in animals; these processes are less well understood in plants. Here, based on characterization of a dwarf rice (Oryza sativa) mutant (dwarf-related wd40 protein 1,drw1) deficient for yeastCTF4(CHROMOSOME TRANSMISSION FIDELITY PROTEIN 4), we discovered thatCTF4orthologs in plants use common cellular machinery yet accomplish divergent functional outcomes. Specifically,drw1exhibited no flowering-related phenotypes (as in the putatively orthologousArabidopsis thaliana eol1mutant), but displayed cell cycle arrest and DNA damage responses. Mechanistically, we demonstrate that DRW1 sustains normal cell cycle progression by modulating the expression of cell cycle inhibitorsKIP-RELATED PROTEIN 1(KRP1) andKRP5, and show that these effects are mediated by DRW1 binding their promoters and increasing H3K27me3 levels. Thus, althoughCTF4orthologsENHANCER OF LHP1 1(EOL1) in Arabidopsis andDRW1in rice are both expressed uniquely in dividing cells, commonly interact with several Polycomb complex subunits, and promote H3K27me3 deposition, we now know that their regulatory functions diverged substantially during plant evolution. Moreover, our work experimentally illustrates specific targets of CTF4/EOL1/DRW1, their protein–proteininteraction partners, and their chromatin/epigenetic effects in plants.